Evidence map›Paper›PMID 35640101›Full record

ArticleJournal of the American Society for Mass Spectrometry2022

Home-Built Spinning Apparatus for Drying Agarose-Based Imaging Mass Spectrometry Samples.

Hannah J Lusk, Sarah E Levy, Tova M Bergsten, Joanna E Burdette, Laura M Sanchez

Open access · greenAbstract read
In one paragraph

Article in Journal of the American Society for Mass Spectrometry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.8field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
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  4. Article
  5. Article
  6. Article
  7. Review
  8. Unveiling a CAAX Protease-Like Protein Involved in Didemnin Drug Maturation and Secretion.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024
    Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Hannah J LuskDepartment of Chemistry and Biochemistry, University of California Santa Cruz, 1156 High Street, Santa Cruz, California 95064, United States.
Sarah E LevyDepartment of Chemistry and Biochemistry, University of California Santa Cruz, 1156 High Street, Santa Cruz, California 95064, United States.
Tova M BergstenDepartment of Pharmaceutical Sciences, University of Illinois at Chicago College of Pharmacy, 900 South Ashland Avenue, Chicago, Illinois 60607, United States.
Joanna E BurdetteDepartment of Pharmaceutical Sciences, University of Illinois at Chicago College of Pharmacy, 900 South Ashland Avenue, Chicago, Illinois 60607, United States.ORCID 0000-0002-7271-6847
Laura M SanchezDepartment of Chemistry and Biochemistry, University of California Santa Cruz, 1156 High Street, Santa Cruz, California 95064, United States.ORCID 0000-0001-9223-7977
University of California, Santa Cruz · USUniversity of Illinois Chicago · US

Funding

Imaging mass spectrometry methodologies for studying the metabolites of cancer metastasisR01CA240423 · NCI · UNIVERSITY OF ILLINOIS AT CHICAGO · PI BURDETTE, JOANNA E, SANCHEZ, LAURA MARGARET · 2020 to 2024
$2.1M
MALDI-TOF MS/IDBac to discover antibiotics from the Great Lakes sponge microbiomeR01GM125943 · NIGMS · UNIVERSITY OF ILLINOIS AT CHICAGO · PI MURPHY, BRIAN THACHER, SANCHEZ, LAURA MARGARET · 2018 to 2021
$2.1M
Regulation of primary metastasis in high grade serous ovarian cancerF30CA260791 · NCI · UNIVERSITY OF ILLINOIS AT CHICAGO · PI BERGSTEN, TOVA · 2021 to 2024
$209k
NCI NIH HHS F30 CA260791NCI NIH HHS R01 CA240423NIGMS NIH HHS R01 GM125943
6 · The paper itself

Abstract

Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) is a useful technique for mapping the spatial distribution of molecules across biological samples. Sample preparation is crucial for MALDI-IMS; samples must be flat, dry, and cocrystallized with a matrix prior to analysis. Agarose-based samples can be difficult to consistently prepare as they are susceptible to environmental changes, which can lead to inconsistent drying and wrinkling on the sample surface. Small height differences may cause low ionization of target analytes or introduce artifacts in imaging data depending on the instrument used for analysis. To overcome the variations, a home-built robotic spinner was constructed and applied to agarose-based samples. This robotic spinner is inexpensive and easy to assemble, and when it was applied to agarose-based samples, accelerated the drying process and reduced wrinkles, improving the overall quality of the resulting IMS data.

Indexed as

Specimen HandlingSepharoseSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSepharose3D cell cultureagaroseimaging mass spectrometryMALDIsample preparation

Identifiers

PMID35640101
PMCPMC9262851
OpenAlexW4281770661

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.