ArticleProceedings of the National Academy of Sciences of the United States of America2022
Genome-wide association identifies a missing hydrolase for tocopherol synthesis in plants.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- A century of vitamin E research: The innovative journey from basic biology to synthetic bio-manufacturing.Journal of integrative plant biology · 2026Review
- An alternative splicing caused by a natural variation in BnaC02.VTE4 gene affects vitamin E and glucosinolate content in rapeseed (Brassica napus L.).Plant biotechnology journal · 2025Article
- Vitamin E in Plants: Biosynthesis Pathways, Biofortification Strategies, and Regulatory Dynamics.International journal of molecular sciences · 2025Review
- Regulatory and retrograde signaling networks in the chlorophyll biosynthetic pathway.Journal of integrative plant biology · 2025Review
- Total tocopherol levels in maize grain depend on chlorophyll biosynthesis within the embryo.BMC plant biology · 2025Article
- A family of α/β hydrolases removes phytol from chlorophyll metabolites for tocopherol biosynthesis in Arabidopsis.The Plant cell · 2025Article
- Dynamic identification of candidate genes associated with higher tocopherol biosynthesis inFrontiers in plant science · 2025Article
- Heterologous Expression of SunflowerPlants (Basel, Switzerland) · 2024Article
- Multi-omics identification of a key glycosyl hydrolase gene FtGH1 involved in rutin hydrolysis in Tartary buckwheat (Fagopyrum tataricum).Plant biotechnology journal · 2024Article
- Tocopherol and phylloquinone biosynthesis in chloroplasts requires the phytol kinase VITAMIN E PATHWAY GENE5 (VTE5) and the farnesol kinase (FOLK).The Plant cell · 2024Article
- Article
- The light and hypoxia induced gene ZmPORB1 determines tocopherol content in the maize kernel.Science China. Life sciences · 2024Article
- Chemical-tag-based semi-annotated metabolomics facilitates gene identification and specialized metabolic pathway elucidation in wheat.The Plant cell · 2024Article
- Genome-wide association study and genotypic variation for the major tocopherol content in rice grain.Frontiers in plant science · 2024Article
- Genome-wide scan for oil quality reveals a coregulation mechanism of tocopherols and fatty acids in soybean seeds.Plant communications · 2023Article
- Combining GWAS and TWAS to identify candidate causal genes for tocochromanol levels in maize grain.Genetics · 2022Article
- Genome-wide association studies reveal novel QTLs, QTL-by-environment interactions and their candidate genes for tocopherol content in soybean seed.Frontiers in plant science · 2022Article
- Vitamin E synthesis and response in plants.Frontiers in plant science · 2022Review
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13 authors.
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Abstract
The tocopherol biosynthetic pathway, encoded by VTE genes 1 through 6, is highly conserved in plants but most large effect quantitative trait loci for seed total tocopherols (totalT) lack VTE genes, indicating other activities are involved. A genome-wide association study of Arabidopsis seed tocopherols showed five of seven significant intervals lacked VTE genes, including the most significant, which mapped to an uncharacterized, seed-specific, envelope-localized, alpha/beta hydrolase with esterase activity, designated AtVTE7. Atvte7 null mutants decreased seed totalT 55% while a leaky allele of the maize ortholog, ZmVTE7, decreased kernel and leaf totalT 38% and 49%, respectively. Overexpressing AtVTE7 or ZmVTE7 partially or fully complemented the Atvte7 seed phenotype and increased leaf totalT by 3.6- and 6.9-fold, respectively. VTE7 has the characteristics of an esterase postulated to provide phytol from chlorophyll degradation for tocopherol synthesis, but bulk chlorophyll levels were unaffected in vte7 mutants and overexpressing lines. Instead, levels of specific chlorophyll biosynthetic intermediates containing partially reduced side chains were impacted and strongly correlated with totalT. These intermediates are generated by a membrane-associated biosynthetic complex containing protochlorophyllide reductase, chlorophyll synthase, geranylgeranyl reductase (GGR) and light harvesting-like 3 protein, all of which are required for both chlorophyll and tocopherol biosynthesis. We propose a model where VTE7 releases prenyl alcohols from chlorophyll biosynthetic intermediates, which are then converted to the corresponding diphosphates for tocopherol biosynthesis.
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