Evidence map›Paper›PMID 35638916›Full record

ArticleMicrobiology spectrum2022

Imaging Minimal Bacteria at the Nanoscale: a Reliable and Versatile Process to Perform Single-Molecule Localization Microscopy in Mycoplasmas.

Fabien Rideau, Audrey Villa, Pauline Belzanne, Emeline Verdier, Eric Hosy, Yonathan Arfi

Open access · goldAbstract read
In one paragraph

Article in Microbiology spectrum, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.0field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 15 citations in OpenAlex.

  1. Mesolens imaging in microbiology.Essays in biochemistry · 2026
    Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Dynamics of chromosome organization in a minimal bacterial cell.Frontiers in cell and developmental biology · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Fabien RideauUniversité de Bordeaux, INRAE, Biologie du Fruit et Pathologie, UMR 1332, Villenave d'Ornon, France.
Audrey VillaUniversité de Bordeaux, INRAE, Biologie du Fruit et Pathologie, UMR 1332, Villenave d'Ornon, France.
Pauline BelzanneInterdisciplinary Institute for Neuroscience, CNRS, Université de Bordeaux, IINS, UMR 5297, Bordeaux, France.
Emeline VerdierInterdisciplinary Institute for Neuroscience, CNRS, Université de Bordeaux, IINS, UMR 5297, Bordeaux, France.
Eric HosyInterdisciplinary Institute for Neuroscience, CNRS, Université de Bordeaux, IINS, UMR 5297, Bordeaux, France.
Yonathan ArfiUniversité de Bordeaux, INRAE, Biologie du Fruit et Pathologie, UMR 1332, Villenave d'Ornon, France.ORCID 0000-0002-6064-7899
Centre National de la Recherche Scientifique · FRUniversité de Bordeaux · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mycoplasmas are the smallest free-living organisms. These bacteria are important models for both fundamental and synthetic biology, owing to their highly reduced genomes. They are also relevant in the medical and veterinary fields, as they are pathogenic to both humans and most livestock species. Mycoplasma cells have minute sizes, often in the 300- to 800-nm range. As these dimensions are close to the diffraction limit of visible light, fluorescence imaging in mycoplasmas is often poorly informative. Recently developed superresolution imaging techniques can break this diffraction limit, improving the imaging resolution by an order of magnitude and offering a new nanoscale vision of the organization of these bacteria. These techniques have, however, not been applied to mycoplasmas before. Here, we describe an efficient and reliable protocol to perform

Indexed as

MycoplasmaSingle Molecule ImagingFluorescent DyesHumansMicroscopyFluorescent DyesdSTORMMycoplasmaPALMsingle-molecule localization microscopysuperresolution microscopy

Identifiers

PMID35638916
PMCPMC9241803
OpenAlexW4282036255

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.