ArticleParasite (Paris, France)2022
Comparison of molecular diagnostic approaches for the detection and differentiation of the intestinal protist Blastocystis sp. in humans.
Article in Parasite (Paris, France), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- Effects of Lactiplantibacillus plantarum and Lacticaseibacillus paracasei supplementation on the single-cell fecal parasitome in children with celiac disease autoimmunity: a randomized, double-blind placebo-controlled clinical trial.Parasites & vectors · 2023Trial
- Molecular Detection of Enteric Protozoa in Cattle from North Portugal.Pathogens (Basel, Switzerland) · 2026Article
- Occurrence and subtyping of the intestinal protozoanFood and waterborne parasitology · 2026Article
- Next-Gen intestinal parasite detection: Leveraging metataxonomics for improved diagnosis of intestinal protists and helminths.PloS one · 2026Article
- Epidemiology ofOne health (Amsterdam, Netherlands) · 2025Article
- Commensal, pathogen, or passenger? Rethinking the role ofOne health (Amsterdam, Netherlands) · 2025Review
- Assessment of the Therapeutic Role of Allium tuncelianum Extract in Rats Infected with Blastocystis Subtype 3.Acta parasitologica · 2025Article
- Aspects of Genetic Diversity, Host Specificity and Public Health Significance of Single-Celled Intestinal Parasites Commonly Observed in Humans and Mostly Referred to as 'Non-Pathogenic'.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2025Review
- Comprehensive Tools for Culturing Blastocystis: A Standardized Resource for Research and Diagnostics.Current protocols · 2025Article
- Shaping the human gut microbiota: The role of canine companionship, lifestyle choices, andOne health (Amsterdam, Netherlands) · 2025Article
- Article
- A cross-sectional survey ofOne health (Amsterdam, Netherlands) · 2024Article
- Asymptomatic carriage of intestinal protists is common in children in Lusaka Province, Zambia.PLoS neglected tropical diseases · 2024Article
- Unveiling Blastocystis epidemiology in Morocco: subtype diversity among clinical patients with and without gastrointestinal manifestations in the Meknes region.Parasitology research · 2024Article
- The opportunistic protist,Emerging microbes & infections · 2023Article
- A Cross-Sectional Study on the Occurrence of the Intestinal Protist,International journal of molecular sciences · 2022Article
- Molecular Detection and Subtype Characterization ofIranian journal of parasitologyArticle
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 4 countries.
Funding
Abstract
Blastocystis is the most commonly found intestinal protist in the world. Accurate detection and differentiation of Blastocystis including its subtypes (arguably species) are essential to understand its epidemiology and role in human health. We compared (i) the sensitivity of conventional PCR (cPCR) and qPCR in a set of 288 DNA samples obtained from stool samples of gut-healthy individuals, and (ii) subtype diversity as detected by next-generation sequencing (NGS) versus Sanger sequencing. Real-time PCR resulted in more positive samples than cPCR, revealing high fecal load of Blastocystis based on the quantification curve in most samples. In subtype detection, NGS was largely in agreement with Sanger sequencing but showed higher sensitivity for mixed subtype colonization within one host. This fact together with use of the combination of qPCR and NGS and obtaining information on the fecal protist load will be beneficial for epidemiological and surveillance studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.