ArticleCell reports methods2022
DIPA-CRISPR is a simple and accessible method for insect gene editing.
Article in Cell reports methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- Infectious transgenesis in arthropods.bioRxiv : the preprint server for biology · 2026Article
- CRISPR-Cas9 mediated knockout of the white gene in the bluetongue virus vector, Culicoides sonorensis (biting midge).Scientific reports · 2026Article
- A genome sequence and efficient CRISPR/Cas9 gene editing tools for the solanaceous specialist pest Tetranychus evansi.Insect biochemistry and molecular biology · 2026Article
- Harnessing CRISPR-Cas technology for insect pest control: current advances and future perspectives.Molecular biology reports · 2026Review
- Knockout of nAChR subunits in spider mites and their phytoseiid predators confers spinosyn cross-resistance and reveals a conserved mode of action in mites.Insect biochemistry and molecular biology · 2026Article
- HUH-tagged Cas9 as a platform for efficient ssODN-mediated knock-in via embryo and adult injection in insects.Communications biology · 2026Article
- Using DIPA-CRISPR for simple and efficient endogenous protein tagging in insects.Cell reports methods · 2026Article
- SYNCAS-mediated CRISPR-Cas9 genome editing in the Jewel wasp, Nasonia vitripennis.Insect molecular biology · 2026Article
- Oogenesis of the German Cockroach: From Morphology to Regulation.Results and problems in cell differentiation · 2026Review
- New developments in biotechnology applied to animals: An assessment of the adequacy and sufficiency of current EFSA guidance for animal risk assessment.EFSA journal. European Food Safety Authority · 2025Article
- Transferable approaches to CRISPR-Cas9 induced genome editing in non-model insects: a brief guide.Frontiers in zoology · 2025Review
- Refined CRISPR/Cas9 genome editing in the pea aphid uncovers the essential roles of Laccase2 in overwintering egg adaptation.PLoS genetics · 2025Article
- Identification of ecdysteroids and ecdysteroidogenic genes in dragonflies and damselflies.Scientific reports · 2025Article
- Spider Eye Development Editing and Silk Fiber Engineering Using CRISPR-Cas.Angewandte Chemie (International ed. in English) · 2025Article
- CRISPR/Cas9-Mediated Knockout of the White Gene inInternational journal of molecular sciences · 2025Article
- The mosaicism of Cas-induced mutations and pleiotropic effects of scarlet gene in an emerging model system.Heredity · 2025Article
- CRISPR/Cas Technology in Insect Insecticide Resistance.Insects · 2025Review
- SYNCAS based CRISPR-Cas9 gene editing in predatory mites, whiteflies and stinkbugs.Insect biochemistry and molecular biology · 2025Article
- Genetic Underpinnings of Cuticular Hydrocarbon Biosynthesis in the German Cockroach, Blattella germanica (L.): Progress and Perspectives.Journal of chemical ecology · 2024Review
- Gill regeneration in the mayflyOpen biology · 2024Article
Corrections and comments
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Authors and funding
4 authors.
Funding
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Abstract
Current approaches for insect gene editing require microinjection of materials into early embryos. This severely limits the application of gene editing to a great number of insect species, especially to those whose reproduction systems preclude access to early embryos for injection. To overcome these limitations, we report a simple and accessible method for insect gene editing, termed "direct parental" CRISPR (DIPA-CRISPR). We show that injection of Cas9 ribonucleoproteins (RNPs) into the haemocoel of adult females efficiently introduces heritable mutations in developing oocytes. Importantly, commercially available standard Cas9 protein can be directly used for DIPA-CRISPR, which makes this approach highly practical and feasible. DIPA-CRISPR enables highly efficient gene editing in the cockroaches, on which conventional approaches cannot be applied, and in the model beetle
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