ArticleNature methods2022
Label-free nanofluidic scattering microscopy of size and mass of single diffusing molecules and nanoparticles.
Article in Nature methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- Article
- Light Scattering Contrast Inversion of Single Metal Nanoparticles Inside a Nanofluidic Channel.The journal of physical chemistry. C, Nanomaterials and interfaces · 2026Article
- Beyond the bulk: engineering analytical functions with nanofluidics and nanostructures.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2026Article
- Illuminating the Interface: In Situ Optical Insights Into Two-Electron ORR Pathways for HAdvanced materials (Deerfield Beach, Fla.) · 2026Review
- An integrated nanophotonic SPRM microsystem for real-time, label-free quantification of single-cell adhesion dynamics.Microsystems & nanoengineering · 2026Article
- Doppler-encoded Mie scattering rainbow of flying particles.Science advances · 2026Article
- The updated role of exosomes in cancer diagnosis and therapy.Discover oncology · 2026Review
- Molecule by Molecule Characterization of a Polymer Molecular Mass Distribution via Mass Photometry.Angewandte Chemie (International ed. in English) · 2026Article
- Label-Free Optical Differentiation of Single Diffusing Amino Acids at Picomolar Concentrations.Research square · 2026Article
- Label-free mass and size characterization of few-kDa biomolecules by hierarchical vision transformer augmented nanofluidic scattering microscopy.Nature communications · 2026Article
- Meta-amplified dark-field interferometric scattering microscopy.Nature communications · 2026Article
- A temperature-controlled chip holder with integrated electrodes for nanofluidic scattering spectroscopy on highly integrated nanofluidic systems.Microsystems & nanoengineering · 2026Article
- Femtoliter Batch Reactors for Nanofluidic Scattering Spectroscopy Analysis of Catalytic Reactions on Single Nanoparticles.Small methods · 2026Article
- Article
- Optical detection of single sub-15 nm objects using elastic scattering strong coupling.Nature communications · 2025Article
- Multiparametric Characterization of Individual Suspended Nanoparticles Using Confocal Fluorescence and Interferometric Scattering Microscopy with Microfluidic Confinement.Nano letters · 2025Article
- Multiparametric functional characterization of individual lipid nanoparticles using surface-sensitive light-scattering microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- 3D nanoprinted fiber-interfaced hollow-core waveguides for high-accuracy nanoparticle tracking analysis.Light, science & applications · 2025Article
- The Emergence of Nanofluidics for Single-Biomolecule Manipulation and Sensing.Analytical chemistry · 2025Review
- Review
Corrections and comments
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Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Label-free characterization of single biomolecules aims to complement fluorescence microscopy in situations where labeling compromises data interpretation, is technically challenging or even impossible. However, existing methods require the investigated species to bind to a surface to be visible, thereby leaving a large fraction of analytes undetected. Here, we present nanofluidic scattering microscopy (NSM), which overcomes these limitations by enabling label-free, real-time imaging of single biomolecules diffusing inside a nanofluidic channel. NSM facilitates accurate determination of molecular weight from the measured optical contrast and of the hydrodynamic radius from the measured diffusivity, from which information about the conformational state can be inferred. Furthermore, we demonstrate its applicability to the analysis of a complex biofluid, using conditioned cell culture medium containing extracellular vesicles as an example. We foresee the application of NSM to monitor conformational changes, aggregation and interactions of single biomolecules, and to analyze single-cell secretomes.
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