ArticleNature methods2022
Ab initio phasing macromolecular structures using electron-counted MicroED data.
Article in Nature methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- Characterization of energy filtering slit widths for MicroED data collection.Journal of structural biology: X · 2026Article
- Practical crystallography with a transmission electron microscope.Journal of microscopy · 2026Article
- Towards time-resolved MicroED grid preparation using mix-and-inject gas dynamic virtual nozzles.IUCrJ · 2026Article
- Membrane protein structure and dynamics probed by MicroED.Biochemical Society transactions · 2026Review
- Advances of 3D microcrystals electron diffraction for transmembrane protein structure determination.Biophysical journal · 2026Review
- Single-cell structural biology with intracellular electron crystallography.Nature communications · 2026Article
- Direct from the seed: an atomic resolution protein structure by ab initio MicroED.Nature communications · 2026Article
- Article
- Strategies for mitigating radiation damage and improving data completeness in 3D electron diffraction of protein crystals.Acta crystallographica. Section D, Structural biology · 2026Article
- Standards for MicroED.Acta crystallographica. Section C, Structural chemistry · 2025Article
- Ligand Screening and Discovery Using Cocktail Soaking and Automated Microcrystal Electron Diffraction.ChemMedChem · 2025Article
- Review
- Article
- Comprehensive microcrystal electron diffraction sample preparation for cryo-EM.Nature protocols · 2025Review
- Recovering high-resolution information using energy filtering in MicroED.Structural dynamics (Melville, N.Y.) · 2025Article
- Structure of the lens MP20 mediated adhesive junction.Nature communications · 2025Article
- Energy filtering enables macromolecular MicroED data at sub-atomic resolution.Nature communications · 2025Article
- Fast event-based electron counting for small-molecule structure determination by MicroED.Acta crystallographica. Section C, Structural chemistry · 2025Article
- Ligand Screening and Discovery using Cocktail Soaking and Automated MicroED.bioRxiv : the preprint server for biology · 2025Article
- 3D Electron Diffraction Structure of an Organic Semiconductor Reveals Conformational Polymorphism.Journal of the American Chemical Society · 2025Article
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Abstract
Structures of two globular proteins were determined ab initio using microcrystal electron diffraction (MicroED) data that were collected on a direct electron detector in counting mode. Microcrystals were identified using a scanning electron microscope (SEM) and thinned with a focused ion beam (FIB) to produce crystalline lamellae of ideal thickness. Continuous-rotation data were collected using an ultra-low exposure rate to enable electron counting in diffraction. For the first sample, triclinic lysozyme extending to a resolution of 0.87 Å, an ideal helical fragment of only three alanine residues provided initial phases. These phases were improved using density modification, allowing the entire atomic structure to be built automatically. A similar approach was successful on a second macromolecular sample, proteinase K, which is much larger and diffracted to a resolution of 1.5 Å. These results demonstrate that macromolecules can be determined to sub-ångström resolution by MicroED and that ab initio phasing can be successfully applied to counting data.
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