Evidence map›Paper›PMID 35635710›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

High-Throughput Imaging of Bacillus subtilis.

Paula Montero Llopis, Ryan Stephansky, Xindan Wang

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Paula Montero LlopisMicRoN Core, Harvard Medical School, Boston, MA, USA.
Ryan StephanskyMicRoN Core, Harvard Medical School, Boston, MA, USA.
Xindan WangDepartment of Biology, Indiana University, Bloomington, IN, USA. xindan@indiana.edu.
Harvard University · USIndiana University Bloomington · US

Funding

Chromosome Dynamics in Bacillus SubtillsR01GM086466 · NIGMS · HARVARD MEDICAL SCHOOL · PI RUDNER, DAVID Z · 2009 to 2021
$4.2M
How bacterial SMC complexes organize chromosomes (Equipment Supplement)R01GM141242 · NIGMS · TRUSTEES OF INDIANA UNIVERSITY · PI WANG, XINDAN · 2021 to 2025
$1.8M
Cell Envelope Homeostasis in Bacillus subtilisR01GM127399 · NIGMS · HARVARD MEDICAL SCHOOL · PI RUDNER, DAVID Z · 2019 to 2022
$1.4M
NIGMS NIH HHS R01 GM086466NIGMS NIH HHS R01 GM127399NIGMS NIH HHS R01 GM141242
6 · The paper itself

Abstract

Bacillus subtilis is a widely used model bacterium to study cellular processes and development. The availability of an arrayed mutant library gave us the opportunity to cytologically analyze every mutant and screen for new genes involved in cell shape determination, cell division, and chromosome segregation. Here we describe a high-throughput method to image arrayed B. subtilis mutant libraries using wide-field fluorescence microscopy. We provide a detailed description of growing the arrayed strain collection, preparing slides containing agarose pedestals, setting up the microscopy procedure, acquiring images, and analyzing the images.

Indexed as

Bacillus subtilisChromosome SegregationCell DivisionGene LibraryMicroscopy, FluorescenceArrayed mutant libraryAutomated imagingBacillus subtilisFluorescence microscopyHigh-throughput imaging

Identifiers

PMID35635710
OpenAlexW4285259737

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.