Evidence map›Paper›PMID 35635705›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Copy Number Analysis of the Yeast Histone Deacetylase Complex Component Cti6 Directly in Living Cells.

Sviatlana Shashkova, Thomas Nyström, Mark C Leake

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Sviatlana ShashkovaDepartment of Microbiology and Immunology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden. sviatlana.shashkova@physics.gu.se.
Thomas NyströmDepartment of Microbiology and Immunology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Mark C LeakeDepartments of Physics and Biology, University of York, York, UK.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Proteins are one of the key components of cellular life that play a crucial role in most biological processes. Therefore, quantification of protein copy numbers is essential for revealing and better understanding of cellular behavior and functions. Here we describe a single-molecule fluorescence-based method of protein copy number quantification directly in living cells. This enables quick and reliable estimations and comparison of the protein of interest abundance without implementing large-scale studies.

Indexed as

Saccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsCarrier ProteinsDNA Copy Number VariationsHistone DeacetylasesProteinsCarrier ProteinsCti6 protein, S cerevisiaeHistone DeacetylasesProteinsSaccharomyces cerevisiae ProteinsCopy numberHistone deacetylaseLiving cellsSingle-molecule

Identifiers

PMID35635705

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.