Evidence map›Paper›PMID 35632815›Full record

ArticleViruses2022

Molecular Analysis of SARS-CoV-2 Lineages in Armenia.

Diana Avetyan, Siras Hakobyan, Maria Nikoghosyan, Lilit Ghukasyan, Gisane Khachatryan, Tamara Sirunyan, Nelli Muradyan, Roksana Zakharyan, Andranik Chavushyan, Varduhi Hayrapetyan and 11 more

Open access · goldAbstract read
In one paragraph

Article in Viruses, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
1.0field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it, 10 citations in OpenAlex.

  1. Pooled it
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  7. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors at 4 institutions in 3 countries.

Diana AvetyanLaboratory of Human Genomics, Institute of Molecular Biology NAS RA, Yerevan 0014, Armenia.ORCID 0000-0002-0126-7474
Siras HakobyanBioinformatics Group, Institute of Molecular Biology NAS RA, Yerevan 0014, Armenia.ORCID 0000-0002-6875-2482
Maria NikoghosyanInstitute of Biomedicine and Pharmacy, Russian-Armenian University, Yerevan 0051, Armenia.ORCID 0000-0002-9598-6524
Lilit GhukasyanLaboratory of Human Genomics, Institute of Molecular Biology NAS RA, Yerevan 0014, Armenia.
Gisane KhachatryanLaboratory of Human Genomics, Institute of Molecular Biology NAS RA, Yerevan 0014, Armenia.
Tamara SirunyanLaboratory of Human Genomics, Institute of Molecular Biology NAS RA, Yerevan 0014, Armenia.
Nelli MuradyanLaboratory of Human Genomics, Institute of Molecular Biology NAS RA, Yerevan 0014, Armenia.
Roksana ZakharyanLaboratory of Human Genomics, Institute of Molecular Biology NAS RA, Yerevan 0014, Armenia.
Andranik ChavushyanLaboratory of Human Genomics, Institute of Molecular Biology NAS RA, Yerevan 0014, Armenia.
Varduhi HayrapetyanLaboratory of Human Genomics, Institute of Molecular Biology NAS RA, Yerevan 0014, Armenia.
Anahit HovhannisyanInstitute of Biomedicine and Pharmacy, Russian-Armenian University, Yerevan 0051, Armenia.
Shah A Mohamed BakhashDepartment of Laboratory Medicine and Pathology, University of Washington, Seattle, WA 98102, USA.ORCID 0000-0003-3170-0862
Keith R JeromeDepartment of Laboratory Medicine and Pathology, University of Washington, Seattle, WA 98102, USA.
Pavitra RoychoudhuryDepartment of Laboratory Medicine and Pathology, University of Washington, Seattle, WA 98102, USA.ORCID 0000-0002-4567-8232
Alexander L GreningerDepartment of Laboratory Medicine and Pathology, University of Washington, Seattle, WA 98102, USA.ORCID 0000-0002-7443-0527
Lyudmila NiazyanNORK Infection Clinical Hospital, MoH RA, Yerevan 0047, Armenia.
Mher DavidyantsNORK Infection Clinical Hospital, MoH RA, Yerevan 0047, Armenia.
Gayane Melik-AndreasyanNational Center of Disease Control and Prevention, Ministry of Health RA, Yerevan 0025, Armenia.
Shushan SargsyanNational Center of Disease Control and Prevention, Ministry of Health RA, Yerevan 0025, Armenia.
Lilit NersisyanArmenian Bioinformatics Institute, Yerevan 0014, Armenia.
Arsen ArakelyanLaboratory of Human Genomics, Institute of Molecular Biology NAS RA, Yerevan 0014, Armenia.ORCID 0000-0002-6851-1056
Russian-Armenian University · AMInstitute of Molecular Biology · AMUniversity of Washington · USScience for Life Laboratory · SE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The sequencing of SARS-CoV-2 provides essential information on viral evolution, transmission, and epidemiology. In this paper, we performed the whole-genome sequencing of SARS-CoV-2 using nanopore and Illumina sequencing to describe the circulation of the virus lineages in Armenia. The analysis of 145 full genomes identified six clades (19A, 20A, 20B, 20I, 21J, and 21K) and considerable intra-clade PANGO lineage diversity. Phylodynamic and transmission analysis allowed to attribute specific clades as well as infer their importation routes. Thus, the first two waves of positive case increase were caused by the 20B clade, the third peak caused by the 20I (Alpha), while the last two peaks were caused by the 21J (Delta) and 21K (Omicron) variants. The functional analyses of mutations in sequences largely affected epitopes associated with protective HLA loci and did not cause the loss of the signal in PCR tests targeting ORF1ab and N genes as confirmed by RT-PCR. We also compared the performance of nanopore and Illumina short-read sequencing and showed the utility of nanopore sequencing as an efficient and affordable alternative for large-scale molecular epidemiology research. Thus, our paper describes new data on the genomic diversity of SARS-CoV-2 variants in Armenia in the global context of the virus molecular genomic surveillance.

Indexed as

COVID-19SARS-CoV-2ArmeniaHigh-Throughput Nucleotide SequencingHumansArmeniacoronavirusCOVID-19Illumina sequencingnanopore sequencingSARS-CoV-2whole-genome sequencing

Identifiers

PMID35632815
PMCPMC9142918
OpenAlexW4280559297

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.