ArticleViruses2022
Simultaneous Giant Virus and Virophage Quantification Using Droplet Digital PCR.
Article in Viruses, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 9 citations in OpenAlex.
- Virophage infection mode determines ecological and evolutionary changes in a host-virus-virophage system.The ISME journal · 2024Article
- Evolution of exploitation and replication of giant viruses and virophages.Virus evolution · 2024Article
- Development and clinical validation of a dual ddPCR assay for detecting carbapenem-resistantFrontiers in microbiology · 2024Article
- Updated Virophage Taxonomy and Distinction from Polinton-like Viruses.Biomolecules · 2023Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viruses are an abundant component of aquatic systems, but their detection and quantification remain a challenge. Virophages co-replicate with giant viruses in the shared host cell, and can inhibit the production of new giant virus particles, thereby increasing the survival of the infected host population. Here, we present a protocol for Droplet Digital PCR (ddPCR) to quantify simultaneously giant virus and virophage in a mixed sample, enabling the rapid, culture-free and high throughput detection of virus and virophage. As virophage can be present as free virus particles or integrated into the virus host's genome as well as associated with organic particles, we developed a simple method that enables discrimination between free and particle-associated virophages. The latter include aggregated virophage particles as well as virophage integrated into the host genome. We used, for our experiments, a host-virus-virophage system consisting of
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