ArticleViruses2022
A Novel Sandwich ELASA Based on Aptamer for Detection of Largemouth Bass Virus (LMBV).
Article in Viruses, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed, 30 citations in OpenAlex.
- Development of a Comprehensive Lesion Severity Classification Model for Largemouth Bass (International journal of molecular sciences · 2025Article
- Molecular dynamics simulations reveal mechanistic insights into aptamer-induced structural rearrangements in viral capsid proteins.Journal of computer-aided molecular design · 2025Article
- Innovative Aptamer-HRP Conjugation for Cardiac Troponin I Detection: A Novel ELASA Approach for AMI Diagnostics.International journal of molecular and cellular medicine · 2025Article
- Aptamer based lateral flow biosensor for rapid detection of largemouth bass virus.Frontiers in microbiology · 2025Article
- Genome-Wide Association Study of Resistance to Largemouth Bass Ranavirus (LMBV) inInternational journal of molecular sciences · 2024Article
- New Insights into Aptamers: An Alternative to Antibodies in the Detection of Molecular Biomarkers.International journal of molecular sciences · 2024Review
- Megalocytivirus and Other Members of the FamilyViruses · 2023Review
- Nucleocapsid protein binding DNA aptamers for detection of SARS-COV-2.Current research in biotechnology · 2023Article
- Advanced Techniques for the Intelligent Diagnosis of Fish Diseases: A Review.Animals : an open access journal from MDPI · 2022Review
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Largemouth bass virus (LMBV) is a major viral pathogen in largemouth bass culture, usually causing high mortality and heavy economic losses. Accurate and early detection of LMBV is crucial for diagnosis and control of the diseases caused by LMBV. Previously, we selected the specific aptamers, LA38 and LA13, targeting LMBV by systematic evolution of ligands by exponential enrichment (SELEX). In this study, we further generated truncated LA38 and LA13 (named as LA38s and LA13s) with high specificity and affinities and developed an aptamer-based sandwich enzyme-linked apta-sorbent assay (ELASA) for LMBV diagnosis. The sandwich ELASA showed high specificity and sensitivity for the LMBV detection, without cross reaction with other viruses. The detection limit of the ELASA was as low as 1.25 × 10
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.