Evidence map›Paper›PMID 35631786›Full record

ArticlePlants (Basel, Switzerland)2022

Application of CRISPR/Cas9 System for Efficient Gene Editing in Peanut.

Anjanasree K Neelakandan, David A Wright, Sy M Traore, Xingli Ma, Binita Subedi, Suman Veeramasu, Martin H Spalding, Guohao He

Open access · goldAbstract read
In one paragraph

Article in Plants (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 21 citations in OpenAlex.

  1. Disruption ofVirulence · 2026
    Article
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  8. Progress in genetic engineering and genome editing of peanuts: revealing the future of crop improvement.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024
    Review
  9. Designing future peanut: the power of genomics-assisted breeding.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024
    Review
  10. Review
  11. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Anjanasree K NeelakandanDepartment of Genetics, Development and Cell Biology, Iowa State University, Ames, IA 50011, USA.
David A WrightDepartment of Genetics, Development and Cell Biology, Iowa State University, Ames, IA 50011, USA.
Sy M TraoreDepartment of Agricultural and Environmental Sciences, Tuskegee University, Tuskegee, AL 36088, USA.
Xingli MaCollege of Agronomy, Henan Agricultural University, Zhengzhou 450002, China.
Binita SubediDepartment of Agricultural and Environmental Sciences, Tuskegee University, Tuskegee, AL 36088, USA.
Suman VeeramasuDepartment of Agricultural and Environmental Sciences, Tuskegee University, Tuskegee, AL 36088, USA.
Martin H SpaldingDepartment of Genetics, Development and Cell Biology, Iowa State University, Ames, IA 50011, USA.
Guohao HeDepartment of Agricultural and Environmental Sciences, Tuskegee University, Tuskegee, AL 36088, USA.
Tuskegee University · USIowa State University · USHenan Agricultural University · CN

Funding

United States Department of Agriculture 2018-67014-27572 and 2018-38821-27758
6 · The paper itself

Abstract

Peanuts are an economically important crop cultivated worldwide. However, several limitations restrained its productivity, including biotic/abiotic stresses. CRISPR/Cas9-based gene-editing technology holds a promising approach to developing new crops with improved agronomic and nutritional traits. Its application has been successful in many important crops. However, the application of this technology in peanut research is limited, probably due to the lack of suitable constructs and protocols. In this study, two different constructs were generated to induce insertion/deletion mutations in the targeted gene for a loss of function study. The first construct harbors the regular gRNA scaffold, while the second construct has the extended scaffold plus terminator. The designed gRNA targeting the coding sequence of the

Indexed as

CRISPR/Cas9functional genomic toolsgene editinghairy root transformationmutationpeanut

Identifiers

PMID35631786
PMCPMC9144340
OpenAlexW4281261198

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.