ArticleNutrients2022
Effects of Iron Supplementation on Testicular Function and Spermatogenesis of Iron-Deficient Rats.
Article in Nutrients, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 34 citations in OpenAlex.
- The effect of obesity interventions on male fertility: a systematic review and meta-analysis.Human reproduction update · 2026Pooled it
- The role of iron in normal and impaired testicular function.Andrology · 2026Review
- Male testosterone synthesis disorders: oxidative stress pathways, regulatory mechanisms, potential of antioxidant therapy, and botanical medicine prospects.Asian journal of andrology · 2026Review
- FBXO39 knockdown promotes spermatogenesis impairment by inducing mitochondrial dysfunction and ferroptosis via inhibiting KDM5A ubiquitination and regulating H3K4me3 demethylation.Cell biology and toxicology · 2026Article
- Electromagnetic fields regulate iron metabolism: From mechanisms to applications.Journal of advanced research · 2026Review
- CD71 defines functionally active spermatogonial stem cells with enhanced transplantation potential in mouse testes.Animal cells and systems · 2026Article
- Nanozymes or Spirulina Platensis: Enhancing Sheep Thermo-Tolerance Through Physio-Metabolic, Immune, and Antioxidant Pathways.Biological trace element research · 2025Article
- A multi-omics approach reveals that lotus root polysaccharide iron ameliorates iron deficiency-induced testicular damage by activating PPARγ to promote steroid hormone synthesis.Journal of advanced research · 2025Article
- Immunohistochemical Detection of Iron-Related Proteins in Sertoli Cell-Only Patterns in Canine Testicular Lesions.Animals : an open access journal from MDPI · 2025Article
- Distinct Clusters of Testosterone Levels, Symptoms, and Serum Trace Elements in Young Men: A Cross-Sectional Analysis.Nutrients · 2025Article
- Transferrin Receptor 2 in Canine Testicular Tumors: An Emerging Key Role in Seminomas.Animals : an open access journal from MDPI · 2025Article
- Resveratrol alleviates testicular toxicity induced by anti-PD-1 through regulating the NRF2-SLC7A11-GPX4 pathway.Frontiers in immunology · 2025Article
- Experimental Varicocele Impairs DNA Methylation and Demethylation in Germ Cells, TESE, and Epididymal Spermatozoa, Impacting Active DNA Demethylation in Zygotes.Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
- Preliminary Evidence of the Possible Roles of the Ferritinophagy-Iron Uptake Axis in Canine Testicular Cancer.Animals : an open access journal from MDPI · 2024Article
- Source and level of dietary iron influence semen quality by affecting inflammation, oxidative stress and iron utilization levels in boars.Journal of animal science and biotechnology · 2024Article
- The effect of red blood cell disorders on male fertility and reproductive health.Nature reviews. Urology · 2024Review
- Dysfunction of DMT1 and miR-135b in the gut-testis axis in high-fat diet male mice.Genes & nutrition · 2024Article
- Transcutaneous vagus nerve stimulation ameliorates cardiac abnormalities in chronically stressed rats.Naunyn-Schmiedeberg's archives of pharmacology · 2024Article
- Trace Metal Accumulation in Rats Exposed to Mine Waters: A Case Study, Bor Area (Serbia).Toxics · 2023Article
- Emerging roles of ferroptosis in male reproductive diseases.Cell death discovery · 2023Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Iron deficiency is the most common micronutrient deficiency in the world. Previous studies have shown that iron deficiency increases oxidative stress and decreases antioxidant enzymes, and studies of male infertility indicated that oxidative stress may affect male reproductive functions. The aim of this study was to investigate the effects of iron supplementation on spermatogenesis and testicular functions in iron-deficient rats. Three-week-old male Sprague Dawley (SD) rats were randomly divided into two groups: an iron-adequate control (AI group, 35 ppm FeSO4) and an iron-deficient group (ID group, <5 ppm FeSO4). After three weeks, the iron-deficient group was divided into an original iron-deficient group and five iron-supplemented groups, the latter fed diets containing different doses of FeSO4 (6, 12, 18, 24, and 35 ppm). After five weeks, blood and testis tissue were analyzed. We presented as median (interquartile range, IQR) for continuous measurements and compared their differences using the Kruskal−Wallis test followed by the Mann−Whitney U test among groups. The results showed that as compared with the AI group, the ID group had significantly lower serum testosterone and poorer spermatogenesis (The medians (QR) were 187.4 (185.6−190.8) of AI group vs. 87.5 (85.7−90.4) of ID group in serum testosterone, p < 0.05; 9.3 (8.8−10.6) of AI group vs. 4.9 (3.4−5.4) of ID group in mean testicular biopsy score (MTBS], p < 0.05); iron supplementation reversed the impairment of testis tissue. In the testosterone biosynthesis pathway, iron supplementation improved the lowered protein expressions of hydroxysteroid dehydrogenases caused by iron deficiency. Additionally, decreased activities of glutathione peroxidase and catalase, and increased cleaved-caspase 8 and caspase 3 expression, were found in the iron-deficient rats. The iron-supplemented rats that received > 12 ppm FeSO4 exhibited improvements in antioxidant levels. In conclusion, iron supplementation can abrogate testis dysfunction due to iron deficiency through regulation of the testicular antioxidant capacity.
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