Evidence map›Paper›PMID 35630422›Full record

ArticleMicroorganisms2022

Lignocellulose Fermentation Products Generated by Giant Panda Gut Microbiomes Depend Ultimately on pH Rather than Portion of Bamboo: A Preliminary Study.

Alberto Scoma, Way Cern Khor, Marta Coma, Robert Heyer, Ruben Props, Tim Bouts, Dirk Benndorf, Desheng Li, Hemin Zhang, Korneel Rabaey

Abstract read
In one paragraph

Article in Microorganisms, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Alberto ScomaCenter for Microbial Ecology & Technology (CMET), University of Ghent, Coupure Links 653, 9000 Ghent, Belgium.ORCID 0000-0001-7981-0847
Way Cern KhorCenter for Microbial Ecology & Technology (CMET), University of Ghent, Coupure Links 653, 9000 Ghent, Belgium.
Marta ComaCenter for Microbial Ecology & Technology (CMET), University of Ghent, Coupure Links 653, 9000 Ghent, Belgium.
Robert HeyerBioprocess Engineering, Otto von Guericke University of Magdeburg, Universitätsplatz 2, 39106 Magdeburg, Germany.ORCID 0000-0001-7840-8899
Ruben PropsCenter for Microbial Ecology & Technology (CMET), University of Ghent, Coupure Links 653, 9000 Ghent, Belgium.
Tim BoutsPairi Daiza Foundation, Domaine de Cambron, 7940 Brugelette, Belgium.
Dirk BenndorfBioprocess Engineering, Otto von Guericke University of Magdeburg, Universitätsplatz 2, 39106 Magdeburg, Germany.ORCID 0000-0003-4021-8525
Desheng LiChina Conservation and Research Centre for Giant Panda (CCRCGP), Dujiangyan City 611830, China.
Hemin ZhangChina Conservation and Research Centre for Giant Panda (CCRCGP), Dujiangyan City 611830, China.
Korneel RabaeyCenter for Microbial Ecology & Technology (CMET), University of Ghent, Coupure Links 653, 9000 Ghent, Belgium.ORCID 0000-0001-8738-7778

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Giant pandas feed almost exclusively on bamboo but miss lignocellulose-degrading genes. Their gut microbiome may contribute to their nutrition; however, the limited access to pandas makes experimentation difficult. In vitro incubation of dung samples is used to infer gut microbiome activity. In pandas, such tests indicated that green leaves are largely fermented to ethanol at neutral pH and yellow pith to lactate at acidic pH. Pandas may feed on either green leaves or yellow pith within the same day, and it is unclear how pH, dung sample, fermentation products and supplied bamboo relate to one another. Additionally, the gut microbiome contribution to solid bamboo digestion must be appropriately assessed. Here, gut microbiomes derived from dung samples with mixed colors were used to ferment green leaves, also by artificially adjusting the initial pH. Gut microbiomes digestion of solid lignocellulose accounted for 30-40% of the detected final fermentation products. At pH 6.5, mixed-color dung samples had the same fermentation profile as green dung samples (mainly alcohols), while adjusting the initial pH to 4.5 resulted in the profile of yellow dung samples (mainly lactate). Metaproteomics confirmed that gut microbiomes attacked hemicellulose, and that the panda's alpha amylase was the predominant enzyme (up to 75%).

Indexed as

16S rRNA genealpha amylaseethanolfermentationgiant pandagut microbiomehemicelluloselactic acidmetabolomicsmeta proteomics

Identifiers

PMID35630422
PMCPMC9146640

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.