ArticleMicroorganisms2022
Identification, Biochemical Characterization, and Safety Attributes of Locally Isolated
Article in Microorganisms, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The Effect of Probiotic Lactobacillus Strains on Porphyromonas gingivalis, the Etiological Factor of Periodontal Diseases-A Systematic Review.Probiotics and antimicrobial proteins · 2026Pooled it
- Promiscuous nitrilase from Bacillus subtilis for herbicide degradation and plant growth promotion.Scientific reports · 2026Article
- Article
- Isolation and Characterization of Biosurfactant-Producing Enterobacter hormaechei subsp. hoffmannii MK-DU2 with Potential Application in Bioremediation of Crude Oil Contamination.Applied biochemistry and biotechnology · 2025Article
- Harnessing the Potential of IgY: A Creative Approach for Immunodiagnosis and Immunotherapy Against Viral Diseases of Animals.Current microbiology · 2025Review
- Lactiplantibacillus plantarum as a sustainable solution for monocrotophos degradation and plant growth enhancement.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Article
- Probiotic potential of 'lactobacilli' isolated from traditionally fermented Ethiopian honey wine (Tej).BMC microbiology · 2025Article
- In-vitro assessment of probiotic potential of bacteria isolated from chicken gastrointestinal tract.Antonie van Leeuwenhoek · 2025Article
- Innovative use of indigenousVeterinary world · 2025Article
- The Influence of Gastric Microbiota and Probiotics inBiomedicines · 2024Review
- Developing Robust Probiotic Consortia: A Methodological Optimization Approach.Current microbiology · 2024Article
- Oxygen Transfer Effect on the Growth of Limosilactobacillus reuteri ATCC 53608 and on Its Metabolic Capacity.Current microbiology · 2024Article
- In Vitro Probiotic Characterization of Yeasts with their Postbiotics' Antioxidant Activity and Biofilm Inhibition Capacity.Current microbiology · 2024Article
- A Review of the Nutritional Aspects and Composition of the Meat, Liver and Fat of Buffaloes in the Amazon.Animals : an open access journal from MDPI · 2024Review
- Biodegradation of Keratin Waste by Bacillus velezensis HFS_F2 through Optimized Keratinase Production Medium.Current microbiology · 2024Article
- Clinical trials of probiotics: Current outlook.Food science & nutrition · 2024Article
- Comparing Intradermal (ID) Rabies Vaccination with Conventional IM Regimen on Humoral Response of New Zealand White Rabbits for the Production of Animal-Derived Polyclonal Antibodies.Veterinary medicine international · 2024Article
- Isolation andFrontiers in microbiology · 2024Article
- Characterization ofInternational journal of microbiology · 2024Article
Corrections and comments
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Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The demand of functional foods is on the rise, and researchers are trying to develop nutritious dairy products by using well-characterized strains of bacteria. In this study, we identified locally isolated strains of Lactobacillus fermentum from Bubalus bubalis (Nilli Ravi buffalo) milk and evaluated their potential as probiotics in food products like fermented milk. Fifteen Lactobacillus strains were initially isolated, and only four strains (NMCC-2, NMCC-14, NMCC-17, and NMCC-27) were examined for morphological and biochemical characterizations due to their ability of gas production in Durham tubes. Moreover, these strains were selected for further probiotic characterizations due to their extreme morphological resemblance with lactic acid bacteria for their antimicrobial activity, enzymatic potential, autoaggregation capability, hydrophobicity, and acid and bile tolerance. All selected isolates showed significant probiotic potential. However, NMCC-14 and NMCC-17 strains showed maximum probiotic potential. The isolates (NMCC-2, NMCC-14, NMCC-17, and NMCC-27) were identified as Lactobacillus fermentum utilizing 16S rRNA gene sequencing. The in vivo safety study of NMCC-14 (dose: 1010 CFU/day/mice; 21 days, orally) showed no histological dysfunctions in a mouse model. Pathogenic bacterial enzymes reduced the beneficial bacterial load in the host gastrointestinal tract. These results suggest that the NMCC-14 strain is safe and can be potentially used as a probiotic. Moreover, fermented milk was prepared by using the NMCC-14 strain. The results revealed that NMCC-14 strain-based fermented milk had significantly (p < 0.05) higher protein content (4.4 ± 0.06), water-holding capacity (WHC), and dynamic viscosity as compared to non-fermented milk. The results suggest that L. fermentum NMCC-14 is safe and nontoxic; hence, it can be a beneficial supplement to be used for the development of dairy products to be subjected to further clinical testing.
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