ArticleMicroorganisms2022
A Propidium Monoazide (PMAxx)-Droplet Digital PCR (ddPCR) for the Detection of Viable
Article in Microorganisms, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 19 citations in OpenAlex.
- Optimized Viability-ddPCR with Triton X-100 Enhancement for Selective Detection of LiveJournal of microbiology and biotechnology · 2026Article
- A metagenomic analysis coupled with oligotrophic enrichment approach for detecting specified microorganisms in potable groundwater samples.Frontiers in microbiology · 2025Article
- Point-of-Care Diagnostic System for ViableDiagnostics (Basel, Switzerland) · 2024Article
- Development of a duplex droplet digital PCR assay for the detection ofMicrobiology spectrum · 2024Article
- A flow cytometric assay to detect viability and persistence ofFrontiers in microbiology · 2024Article
- Propidium Monoazide-Treated, Cell-Direct, Quantitative PCR for Detecting Viable Chloramphenicol-ResistantGenes · 2023Article
- Propidium Monoazide (PMAxx)-Recombinase Polymerase Amplification Exo (RPA Exo) Assay for Rapid Detection ofMicroorganisms · 2023Article
- Detection, Genophenotypic Characterization, and Antimicrobial Resistance of Microbial Contaminants.Microorganisms · 2023Article
- Rapid Monitoring of Viable Genetically ModifiedMicroorganisms · 2023Article
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pharmaceutical products contaminated with Burkholderia cepacia complex (BCC) strains constitute a serious health issue for susceptible individuals. New detection methods to distinguish DNA from viable cells are required to ensure pharmaceutical product quality and safety. In this study, we have assessed a droplet digital PCR (ddPCR) with a variant propidium monoazide (PMAxx) for selective detection of live/dead BCC cells in autoclaved nuclease-free water after 365 days, in 0.001% chlorhexidine gluconate (CHX), and in 0.005% benzalkonium chloride (BZK) solutions after 184 days. Using 10 μM PMAxx and 5 min light exposure, a proportion of dead BCC was quantified by ddPCR. The detection limit of culture-based method was 104 CFU/mL, equivalent to 9.7 pg/μL for B. cenocepacia J2315, while that of ddPCR was 9.7 fg/μL. The true positive rate from nuclease-free water and CHX using PMAxx-ddPCR assay was 60.0% and 38.3%, respectively, compared to 85.0% and 74.6% without PMAxx (p < 0.05), respectively. However, in BZK-treated cells, no difference in the detection rate was observed between the ddPCR assay on samples treated with PMAxx (67.1%) and without PMAxx (63.3%). This study shows that the PMAxx-ddPCR assay provides a better tool for selective detection of live BCC cells in non-sterile pharmaceutical products.
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