A General, Label-Free and Homogeneous Electrochemical Strategy for Probing of Protease Activity and Screening of Inhibitor.

Yunxiao Feng, Gang Liu, Fan Zhang, Jianwen Liu, Ming La, Ning Xia

Open access · goldAbstract read

In one paragraph

Article in Micromachines, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.3field-weighted citation impact, top 45% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

Progress in the Development of Biosensors Based on Peptide-Copper Coordination Interaction.Biosensors · 2022
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Yunxiao FengCollege of Chemistry and Chemical Engineering, Pingdingshan University, Pingdingshan 467000, China.

Gang LiuCollege of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou 450001, China.

Fan ZhangCollege of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China.

Jianwen LiuCollege of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China.

Ming LaCollege of Chemistry and Chemical Engineering, Pingdingshan University, Pingdingshan 467000, China.

Ning XiaCollege of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China.ORCID 0000-0003-0366-7524

Anyang Normal University · CNPingdingshan University · CNHenan University of Technology · CN

Funding

the National Natural Science Foundation of China U2004193the Program for Innovative Research Team of Science and Technology in the University of Henan Province 21IRTSTHN005

6 · The paper itself

Abstract

Proteases play a critical role in regulating various physiological processes from protein digestion to wound healing. Monitoring the activity of proteases and screening their inhibitors as potential drug molecules are of great importance for the early diagnosis and treatment of many diseases. In this work, we reported a general, label-free and homogeneous electrochemical method for monitoring protease activity based on the peptide-copper interaction. Cleavage of peptide substrate results in the generation of a copper-binding chelator peptide with a histidine residue in the first or third position (His

Indexed as

angiotensin-converting enzymecopperelectrochemical biosensorproteasethrombin

Identifiers

PMID35630268

DOI10.3390/mi13050803

PMCPMC9148143

OpenAlexW4281251575

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.