Evidence map›Paper›PMID 35628419›Full record

ArticleInternational journal of molecular sciences2022

Microbial Consortia and Mixed Plastic Waste: Pangenomic Analysis Reveals Potential for Degradation of Multiple Plastic Types via Previously Identified PET Degrading Bacteria.

Sabrina Edwards, Rosa León-Zayas, Riyaz Ditter, Helen Laster, Grace Sheehan, Oliver Anderson, Toby Beattie, Jay L Mellies

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed, 2 pooled it
2.7field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 2 syntheses or guidelines pooled it, 55 citations in OpenAlex.

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  16. Biofilm mitigation in hybrid chemical-biological upcycling of waste polymers.Frontiers in bioengineering and biotechnology · 2024
    Article
  17. Article
  18. Biodegradation of Pollutants in the Environment: Omics Approaches.International journal of molecular sciences · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Sabrina EdwardsBiology Department, Reed College, Portland, OR 97202, USA.ORCID 0000-0003-1950-9002
Rosa León-ZayasBiology Department, Willamette University, Salem, OR 97301, USA.
Riyaz DitterBiology Department, Reed College, Portland, OR 97202, USA.ORCID 0000-0002-8082-3204
Helen LasterBiology Department, Reed College, Portland, OR 97202, USA.ORCID 0000-0001-7472-1394
Grace SheehanBiology Department, Willamette University, Salem, OR 97301, USA.
Oliver AndersonBiology Department, Willamette University, Salem, OR 97301, USA.
Toby BeattieBiology Department, Reed College, Portland, OR 97202, USA.
Jay L MelliesBiology Department, Reed College, Portland, OR 97202, USA.ORCID 0000-0001-5654-6481
Reed College · USWillamette University · US

Funding

National Science Foundation 1931150
6 · The paper itself

Abstract

The global utilization of single-use, non-biodegradable plastics, such as bottles made of polyethylene terephthalate (PET), has contributed to catastrophic levels of plastic pollution. Fortunately, microbial communities are adapting to assimilate plastic waste. Previously, our work showed a full consortium of five bacteria capable of synergistically degrading PET. Using omics approaches, we identified the key genes implicated in PET degradation within the consortium's pangenome and transcriptome. This analysis led to the discovery of a novel PETase, EstB, which has been observed to hydrolyze the oligomer BHET and the polymer PET. Besides the genes implicated in PET degradation, many other biodegradation genes were discovered. Over 200 plastic and plasticizer degradation-related genes were discovered through the Plastic Microbial Biodegradation Database (PMBD). Diverse carbon source utilization was observed by a microbial community-based assay, which, paired with an abundant number of plastic- and plasticizer-degrading enzymes, indicates a promising possibility for mixed plastic degradation. Using RNAseq differential analysis, several genes were predicted to be involved in PET degradation, including aldehyde dehydrogenases and several classes of hydrolases. Active transcription of PET monomer metabolism was also observed, including the generation of polyhydroxyalkanoate (PHA)/polyhydroxybutyrate (PHB) biopolymers. These results present an exciting opportunity for the bio-recycling of mixed plastic waste with upcycling potential.

Indexed as

Microbial ConsortiaPolyethylene TerephthalatesBacteriaPlasticizersPlasticsPlasticizersPlasticsPolyethylene Terephthalatesbiodegradationmixed plasticspangenomesplasticizerspoly(ethylene)terephthalate (PET)polyhydroxyalkanoate (PHA)

Identifiers

PMID35628419
PMCPMC9146961
OpenAlexW4280589679

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.