ArticleBiology2022
High-Efficiency Bovine Sperm Sexing Used Magnetic-Activated Cell Sorting by Coupling scFv Antibodies Specific to Y-Chromosome-Bearing Sperm on Magnetic Microbeads.
Article in Biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 23 citations in OpenAlex.
- Systematic review and meta-analysis of cryopreserved bovine sperm assessment: harnessing imaging flow cytometry for multi-parametric analysis.Frontiers in veterinary science · 2024Pooled it
- Sperm Sexing in Selected Animals and Humans: Methods, Applications, and Future Prospects.Andrology · 2026Review
- Electrospun poly(lactic acid) biopolymer fibers functionalized with Y-sperm-specific scFv enable immunoaffinity-driven bovine sperm sexing.Scientific reports · 2026Article
- Enhancement of Y-bearing sperm enrichment in swamp buffalo using a TLR7/8 agonist (R848) combined with discontinuous Percoll density gradient centrifugation.Veterinary world · 2026Article
- The effect of toll-like receptor 7/8 activation by ligand (R848) on morphometry, proportion of X-Y chromosome-bearing sperm, and sexed sperm quality of bucks.Open veterinary journal · 2026Article
- Two decades of sperm sex-sorting in animals: A comprehensive systematic review and meta-analysis of techniques, effectiveness, and global research trends (2005-2025).Veterinary world · 2025Review
- Progress of sex control techniques in mammals†.Biology of reproduction · 2025Review
- Novel single-chain fragment variable antibody targeting plasma membrane epitopes on porcine Y-chromosome-bearing sperm.Scientific reports · 2025Article
- Deciphering aptamer-protein interactions for bovine sperm sorting through in silico and in vitro studies.Molecular biology reports · 2025Article
- Advances in immunological sorting of X and Y chromosome-bearing sperm: from proteome to sex-specific proteins.Frontiers in veterinary science · 2025Review
- Proteomic and Metabolomic Profiling Reveals Alterations in Boar X and Y Sperm.Animals : an open access journal from MDPI · 2024Article
- Exploring aptamers for targeted enrichment of X sperm in bovine: unraveling selective potential.Animal biotechnology · 2024Article
- Article
- The reproductive success of Simmental bovine after sex-sorting under various incubation and centrifugation protocols.Veterinary world · 2023Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
Sperm sexing technique is favored in the dairy industry. This research focuses on the efficiency of bovine sperm sexing using magnetic-activated cell sorting (MACS) by scFv antibody against Y-chromosome-bearing sperm (Y-scFv) coupled to magnetic microbeads and its effects on kinematic variables, sperm quality, and X/Y-sperm ratio. In this study, the optimal concentration of Y-scFv antibody coupling to the surface of magnetic microbeads was 2-4 mg/mL. PY-microbeads revealed significantly enriched Y-chromosome-bearing sperm (Y-sperm) in the eluted fraction (78.01-81.43%) and X-chromosome-bearing sperm (X-sperm) in the supernatant fraction (79.04-82.65%). The quality of frozen-thawed sexed sperm was analyzed by CASA and imaging flow cytometer, which showed that PY-microbeads did not have a negative effect on X-sperm motility, viability, or acrosome integrity. However, sexed Y-sperm had significantly decreased motility and viability. The X/Y-sperm ratio was determined using an imaging flow cytometer and real-time PCR. PY-microbeads produced sperm with up to 82.65% X-sperm in the X-enriched fraction and up to 81.43% Y-sperm in the Y-enriched fraction. Bovine sperm sexing by PY-microbeads showed high efficiency in separating Y-sperm from X-sperm and acceptable sperm quality. This initial technique is feasible for bovine sperm sexing, which increases the number of heifers in dairy herds while lowering production expenses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.