Evidence map›Paper›PMID 35625377›Full record

ArticleBiology2022

Gene Editing of the Catfish Gonadotropin-Releasing Hormone Gene and Hormone Therapy to Control the Reproduction in Channel Catfish,

Guyu Qin, Zhenkui Qin, Cuiyu Lu, Zhi Ye, Ahmed Elaswad, Max Bangs, Hanbo Li, Yiliu Zhang, Yingqi Huang, Huitong Shi and 11 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
3.0field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 16 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors at 9 institutions in 5 countries.

Guyu QinSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
Zhenkui QinSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.ORCID 0000-0002-8717-1210
Cuiyu LuSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
Zhi YeSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
Ahmed ElaswadSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
Max BangsSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
Hanbo LiSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
Yiliu ZhangSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
Yingqi HuangSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
Huitong ShiSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
Kamal GoshSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.ORCID 0000-0003-2979-2526
Nermeen Y AbassSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.ORCID 0000-0003-3590-2269
Khoi VoSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
Ramjie OdinSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
William S BuggSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
Nathan J C BackenstoseSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
David DrescherSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
Zachary TaylorSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
Timothy BradenDepartment of Anatomy, Physiology and Pharmacology, Auburn University, Auburn, AL 36849, USA.
Baofeng SuSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.ORCID 0000-0002-9633-2733
Rex A DunhamSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
Auburn University · USShanghai Ocean University · CNFlorida State University · USLangston University · USMindanao State University · PHOcean University of China · CNSuez Canal University · EGUniversity at Buffalo, State University of New York · USUniversity of Manitoba · CA

Funding

Biotechnology Risk Assessment Grant Program, USDA National Institute of Food and Agriculture and the Agricultural Research Service 2014-33522-22263
6 · The paper itself

Abstract

Transcription activator-like effector nuclease (TALEN) plasmids targeting the channel catfish gonadotropin-releasing hormone (cfGnRH) gene were delivered into fertilized eggs with double electroporation to sterilize channel catfish (Ictalurus punctatus). Targeted cfGnRH fish were sequenced and base deletion, substitution, and insertion were detected. The gene mutagenesis was achieved in 52.9% of P1 fish. P1 mutants (individuals with human-induced sequence changes at the cfGnRH locus) had lower spawning rates (20.0−50.0%) when there was no hormone therapy compared to the control pairs (66.7%) as well as having lower average egg hatch rates (2.0% versus 32.3−74.3%) except for one cfGnRH mutated female that had a 66.0% hatch rate. After low fertility was observed in 2016, application of luteinizing hormone-releasing hormone analog (LHRHa) hormone therapy resulted in good spawning and hatch rates for mutants in 2017, which were not significantly different from the controls (p > 0.05). No exogenous DNA fragments were detected in the genome of mutant P1 fish, indicating no integration of the plasmids. No obvious effects on other economically important traits were observed after the knockout of the reproductive gene in the P1 fish. Growth rates, survival, and appearance between mutant and control individuals were not different. While complete knock-out of reproductive output was not achieved, as these were mosaic P1 brood stock, gene editing of channel catfish for the reproductive confinement of gene-engineered, domestic, and invasive fish to prevent gene flow into the natural environment appears promising.

Indexed as

catfish gonadotropin-releasing hormonechannel catfishhormone therapytranscription activator-like effector nucleases

Identifiers

PMID35625377
PMCPMC9138287
OpenAlexW4224313263

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.