ArticleBiosensors2022
Analyzing Human Periodontal Soft Tissue Inflammation and Drug Responses In Vitro Using Epithelium-Capillary Interface On-a-Chip.
Article in Biosensors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- A review of the wide applications of microfluidic devices in peri-implantitis, periodontitis, and endodontics.Odontology · 2026Review
- Oral tissue spheroid, organoid, and organ-on chip microphysiological modeling strategies towards enhanced emulation of health and disease.Bioengineering & translational medicine · 2025Review
- Engineered Tissue Models to Decode Host-Microbiota Interactions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Review
- Advances in modeling periodontal host-microbe interactions: insights from organotypic and organ-on-chip systems.Lab on a chip · 2025Review
- Underscoring long-term host-microbiome interactions in a physiologically relevant gingival tissue model.NPJ biofilms and microbiomes · 2025Article
- Biomimetic bone-vessel interface-on-a-chip for simulating periodontal physiological and pathological microenvironment.Regenerative biomaterials · 2025Article
- "Periodontal ligament-on-chip" as a Novel Tool for Studies on the Physiology and Pathology of Periodontal Tissues.Advanced healthcare materials · 2024Article
- Bioengineering the Junctional Epithelium in 3D Oral Mucosa Models.Journal of functional biomaterials · 2024Review
- Biofabrication Strategies for Oral Soft Tissue Regeneration.Advanced healthcare materials · 2024Review
- Microfluidic organ-on-chip systems for periodontal research: advances and future directions.Frontiers in bioengineering and biotechnology · 2024Review
- A microfluidic organ-on-a-chip: into the next decade of bone tissue engineering applied in dentistry.Future science OA · 2023Review
- Microphysiological Modeling of Gingival Tissues and Host-Material Interactions Using Gingiva-on-Chip.Advanced healthcare materials · 2023Article
- A physiologically relevant culture platform for long-term studies of in vitro gingival tissue.Acta biomaterialia · 2023Article
- Potential Clinical Application of Organs-on-a-Chip in Periodontal Diseases: A Systematic Review of In Vitro Studies.Dentistry journal · 2023Review
- Gut-on-a-Chip Research for Drug Development: Implications of Chip Design on Preclinical Oral Bioavailability or Intestinal Disease Studies.Biomimetics (Basel, Switzerland) · 2023Review
- Mechanical Regulation of Oral Epithelial Barrier Function.Bioengineering (Basel, Switzerland) · 2023Article
- The Application of Organs-on-a-Chip in Dental, Oral, and Craniofacial Research.Journal of dental research · 2023Review
- Microfluidic Based Organ-on-Chips and Biomedical Application.Biosensors · 2023Article
- In-vitro models of biocompatibility testing for restorative dental materials: From 2D cultures to organs on-a-chip.Acta biomaterialia · 2022Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
The gingival epithelium-capillary interface is a unique feature of periodontal soft tissue, preserving periodontal tissue homeostasis and preventing microorganism and toxic substances from entering the subepithelial tissue. However, the function of the interface is disturbed in periodontitis, and mechanisms of the breakdown of the interface are incompletely understood. To address these limitations, we developed a microfluidic epithelium-capillary barrier with a thin culture membrane (10 μm) that closely mimics the in vivo gingival epithelial barrier with an immune micro-environment. To test the validity of the fabricated gingival epithelial barrier model, epithelium-capillary interface-on-a-chip was cultured with human gingival epithelial cells (HGECs) and human vascular endothelial cells (HUVEC). Their key properties were tested using optical microscope, transepithelial/transendothelial electrical resistance (TEER), and permeability assays. The clear expression of VE-cadherin revealed the tight junctions in endothelial cells. Live/dead assays indicated a high cell viability, and the astrocytic morphology of HGE cells was confirmed by F-actin immunostaining. By the third day of cell culture, TEER levels typically exceeded in co-cultures. The resultant permeability coefficients showed a significant difference between 70 kDa and 40 kDa FITC-dextran. The expression of protein intercellular cell adhesion molecule (ICAM-1) and human beta defensin-2 (HBD2) decreased when exposed to TNF-α and LPS, but recovered with the NF-κB inhibitor treatment- Pyrrolidinedithiocarbamic acid (PDTC), indicating the stability of the fabricated chip. These results demonstrate that the developed epithelium-capillary interface system is a valid model for studying periodontal soft tissue function and drug delivery.
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Registered trials
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