ArticleBiosensors2022
A Label-Free Electrochemical Impedimetric Immunosensor with Biotinylated-Antibody for SARS-CoV-2 Nucleoprotein Detection in Saliva.
Article in Biosensors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 50 citations in OpenAlex.
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- A Microfluidic Platform Integrating Dielectrophoretic Concentration and Impedimetric Sensing for RapidACS sensors · 2025Article
- Machine-Learning-Aided Advanced Electrochemical Biosensors.Advanced materials (Deerfield Beach, Fla.) · 2025Review
- A Sandwich-Type Impedimetric Immunosensor for the Detection of Tau-441 Biomarker.Bioengineering (Basel, Switzerland) · 2025Article
- Electrochemical Impedance Spectroscopy-Based Biosensors for Label-Free Detection of Pathogens.Biosensors · 2025Review
- Review
- Development and Optimization of a Cost-Effective Electrochemical Immunosensor for Rapid COVID-19 Diagnosis.Biosensors · 2025Article
- IgY antibodies/Cysteamine: simple and effective methodology for electrochemical detection of SARS-CoV-2 S-protein.Memorias do Instituto Oswaldo Cruz · 2025Article
- AlHeliyon · 2024Article
- Advances in nanobiosensors during the COVID-19 pandemic and future perspectives for the post-COVID era.Nano convergence · 2024Review
- Reports on the sensitivity enhancement in interferometric based biosensors by biotin-streptavidin system.Heliyon · 2023Article
- Grafting Strategies of Oxidation-Prone Coiled-Coil Peptides for Protein Capture in Bioassays: Impact of Orientation and the Oxidation State.ACS omega · 2023Article
- COVID-19 impedimetric biosensor based on polypyrrole nanotubes, nickel hydroxide and VHH antibody fragment: specific, sensitive, and rapid viral detection in saliva samples.Materials today. Chemistry · 2023Article
- Electrochemical Immunosensor for the Determination of Antibodies against Prostate-Specific Antigen Based on ZnO Nanostructures.International journal of molecular sciences · 2023Article
- Review
- Recent Advances in Electrochemical Immunosensors with Nanomaterial Assistance for Signal Amplification.Biosensors · 2023Review
- A Mediated Enzymatic Electrochemical Sensor Using Paper-Based Laser-Induced Graphene.Biosensors · 2022Article
- Impedimetric Detection Based on Label-Free Immunoassay Developed for Targeting Spike S1 Protein of SARS-CoV-2.Diagnostics (Basel, Switzerland) · 2022Article
- The role of electrochemical biosensors in SARS-CoV-2 detection: a bibliometrics-based analysis and review.RSC advances · 2022Review
- SARS-CoV-2 Vaccination Improves Semen Quality in Men Recovered From COVID-19: A Retrospective Cohort Study.American journal of men's healthArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The timely detecting of SARS-CoV-2 coronavirus antigens for infection validation is an urgent request for COVID-19 pandemic control. This study constructed label-free electrochemical impedance spectroscopy (EIS)-based immunosensors based on gold nanostructured screen-printed carbon electrodes (AuNS/SPCEs) to detect the SARS-CoV-2 nucleocapsid protein (N-protein) in saliva. Using short-chain 3-mercaptopropionic acid (MPA) as a linker to covalently bond streptavidin (SA) and bovine serum albumin (BSA) for controlling the oriented immobilization of the biotinylated anti-N-protein antibody (BioAb) can offer a greater sensitivity, a lower limit of detection (LOD), and better reproducibility of immunosensors (defined as BioAb/SA-BSA/MPA/AuNS/SPCEs) than the antibody randomly immobilized immunosensors and the long-chain 11-mercaptoundecanoic acid (MUA)-modified immunosensors (BioAb/SA-BSA/MUA/AuNS/SPCEs). The BioAb/SA-BSA/MPA/AuNS/SPCE-based immunosensors presented good linearity from 0.01 ng/mL to 100 ng/mL and a low LOD of 6 pg/mL in a phosphate buffer solution (PBS) and PBS-diluted saliva. Moreover, the immunosensor exhibited little cross-activity with other viral antigens such as MERS-CoV N-protein, influenza A N-protein, influenza B N-protein, and SARS-CoV-2 spike protein, indicating the high specificity of the immunosensors. The disposable label-free EIS-based immunosensors have promising potential in facilitating the rapid and sensitive tests of saliva-based COVID-19 diagnostics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.