ArticleBMC veterinary research2022
Utility of ultra-rapid real-time PCR for detection and prevalence of Rickettsia spp. in ticks.
Article in BMC veterinary research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed, 6 citations in OpenAlex.
- Prevalence ofPathogens (Basel, Switzerland) · 2025Article
- Genetic diversity and prevalence of emerging Rickettsiales in Yunnan Province: a large-scale study.Infectious diseases of poverty · 2024Article
- Molecular Identification of Spotted Fever Group Rickettsiae in Ticks in the Republic of Korea.Pathogens (Basel, Switzerland) · 2024Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRickettsia spp. are important tick-borne pathogens that cause various human and animal diseases worldwide. A tool for rapid and accurate detection of the pathogens from its vectors is necessary for prevention of Rickettsioses propagation in humans and animals, which are infested by ticks. Therefore, this study was conducted to evaluate a molecular tool, ultra-rapid real-time PCR (UR-qPCR), for rapid and accurate detection of Rickettsia spp. from 5644 ticks in 408 pools collected from livestock and their surrounding environments in Gangwon and Jeju province in South Korea.
resultsThe UR-qPCR of Rickettsia DNA showed a limit of detection of 2.72 × 10
conclusionsThe UR-qPCR system was demonstrated to be useful tool for accurate and rapid detection of Rickettsia from its vector, ixodid ticks, within 20 min. The data on Rickettsia spp. in ticks detected in this study provide useful information on the distribution of Rickettsia in previously unstudied Korean provinces, which are important for the prevention and control of the spread of rickettsioses in both animals and humans in the country.
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