ArticleNature communications2022
Automated vitrification of cryo-EM samples with controllable sample thickness using suction and real-time optical inspection.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 41 citations in OpenAlex.
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- A practical protocol for correlative confocal fluorescence and transmission electron microscopy characterization of extracellular vesicles.Microbiology spectrum · 2025Article
- Advancing Cryo-EM and Cryo-ET through Innovation in Sample Carriers: A Perspective.Analytical chemistry · 2025Review
- Automated device for rapid sample cooling via controlled submersion.Cryobiology · 2025Article
- RNA sample optimization for cryo-EM analysis.Nature protocols · 2025Review
- Sample Preparation Protocol for Laboratory Cryo-Soft X-Ray Microscopy for Studying Cellular Nanoparticle Uptake.International journal of molecular sciences · 2025Article
- 3DCryoHolder: a new open access 3D printable system to store and transport silicon nitride membranes under cryogenic conditions for spectromicroscopy at low temperature.Journal of synchrotron radiation · 2025Article
- A complete 3D-printed tool kit for Solid-State NMR sample and rotor handling.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2024Article
- CryoEM grid preparation: a closer look at advancements and impact of preparation mode and new approaches.Biochemical Society transactions · 2024Review
- VitroJet: new features and case studies.Acta crystallographica. Section D, Structural biology · 2024Article
- Cryo-electron microscopy-based drug design.Frontiers in molecular biosciences · 2024Review
- Scaling up cryo-EM for biology and chemistry: The journey from niche technology to mainstream method.Structure (London, England : 1993) · 2023Review
- Copper Oxide Spike Grids for Enhanced Solution Transfer in Cryogenic Electron Microscopy.Molecules and cells · 2023Article
- Polyelectrolyte coating of cryo-EM grids improves lateral distribution and prevents aggregation of macromolecules.Acta crystallographica. Section D, Structural biology · 2022Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The speed and efficiency of data collection and image processing in cryo-electron microscopy have increased over the last decade. However, cryo specimen preparation techniques have lagged and faster, more reproducible specimen preparation devices are needed. Here, we present a vitrification device with highly automated sample handling, requiring only limited user interaction. Moreover, the device allows inspection of thin films using light microscopy, since the excess liquid is removed through suction by tubes, not blotting paper. In combination with dew-point control, this enables thin film preparation in a controlled and reproducible manner. The advantage is that the quality of the prepared cryo specimen is characterized before electron microscopy data acquisition. The practicality and performance of the device are illustrated with experimental results obtained by vitrification of protein suspensions, lipid vesicles, bacterial and human cells, followed by imaged using single particle analysis, cryo-electron tomography, and cryo correlated light and electron microscopy.
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