Evidence map›Paper›PMID 35622964›Full record

ArticleBioconjugate chemistry2022

Evaluating the Effect of Dye-Dye Interactions of Xanthene-Based Fluorophores in the Fluorosequencing of Peptides.

James L Bachman, Christopher D Wight, Angela M Bardo, Amber M Johnson, Cyprian I Pavlich, Alexander J Boley, Holden R Wagner, Jagannath Swaminathan, Brent L Iverson, Edward M Marcotte and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioconjugate chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

James L BachmanDepartment of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United States.
Christopher D WightDepartment of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United States.ORCID 0000-0003-3389-1762
Angela M BardoDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712, United States.
Amber M JohnsonDepartment of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United States.
Cyprian I PavlichDepartment of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United States.
Alexander J BoleyDepartment of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United States.
Holden R WagnerDepartment of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United States.
Jagannath SwaminathanDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712, United States.
Brent L IversonDepartment of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United States.ORCID 0000-0001-7974-3605
Edward M MarcotteDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712, United States.
Eric V AnslynDepartment of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United States.ORCID 0000-0002-5137-8797

Funding

Mapping the CPLANE interactome, an extensive protein interaction network underlying human ciliopathiesR01HD085901 · NICHD · UNIVERSITY OF TEXAS AT AUSTIN · PI EDWARD M MARCOTTE, John B Wallingford · 2016 to 2026
$6.2M
Proteomics and model organism humanization to decode human geneticsR35GM122480 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI EDWARD M MARCOTTE · 2017 to 2026
$5.4M
Mapping the blood cell protein complexosomeR01DK110520 · NIDDK · UNIVERSITY OF TEXAS AT AUSTIN · PI EMILI, ANDREW, MARCOTTE, EDWARD M · 2016 to 2018
$727k
NICHD NIH HHS R01 HD085901NIDDK NIH HHS R01 DK110520NIGMS NIH HHS R35 GM122480
6 · The paper itself

Abstract

A peptide sequencing scheme utilizing fluorescence microscopy and Edman degradation to determine the amino acid position in fluorophore-labeled peptides was recently reported, referred to as fluorosequencing. It was observed that multiple fluorophores covalently linked to a peptide scaffold resulted in a decrease in the anticipated fluorescence output and worsened the single-molecule fluorescence analysis. In this study, we report an improvement in the photophysical properties of fluorophore-labeled peptides by incorporating long and flexible (PEG)

Indexed as

Fluorescent DyesXanthenesAmino AcidsAzidesIonophoresPeptidesAmino AcidsAzidesFluorescent DyesIonophoresPeptidesXanthenes

Identifiers

PMID35622964
PMCPMC9759034

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.