Evidence map›Paper›PMID 35622897›Full record

ArticleMicrobial genomics2022

Target-enrichment sequencing yields valuable genomic data for challenging-to-culture bacteria of public health importance.

Tristan P W Dennis, Barbara K Mable, Brian Brunelle, Alison Devault, Ryan W Carter, Clare L Ling, Blandina T Mmbaga, Jo E B Halliday, Katarina Oravcova, Taya L Forde

Open access · goldAbstract read
In one paragraph

Article in Microbial genomics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.1field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
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  5. Typing ofMicroorganisms · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 4 countries.

Tristan P W DennisInstitute of Biodiversity, Animal Health & Comparative Medicine, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
Barbara K MableInstitute of Biodiversity, Animal Health & Comparative Medicine, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
Brian BrunelleDaicel Arbor Biosciences, Ann Arbor, Michigan, USA.
Alison DevaultDaicel Arbor Biosciences, Ann Arbor, Michigan, USA.
Ryan W CarterInstitute of Biodiversity, Animal Health & Comparative Medicine, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
Clare L LingShoklo Malaria Research Unit, Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Mae Sot, Thailand.
Blandina T MmbagaKilimanjaro Clinical Research Institute, Kilimanjaro Christian Medical Centre, Moshi, Tanzania.
Jo E B HallidayInstitute of Biodiversity, Animal Health & Comparative Medicine, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
Katarina OravcovaInstitute of Biodiversity, Animal Health & Comparative Medicine, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
Taya L FordeInstitute of Biodiversity, Animal Health & Comparative Medicine, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
University of Glasgow · GBAastrom Biosciences (United States) · USKilimanjaro Christian Medical Centre · TZMahidol University · TH

Funding

Biotechnology and Biological Sciences Research Council BB/R012075/1Medical Research Council MC_PC_16045Wellcome Trust
6 · The paper itself

Abstract

Genomic data contribute invaluable information to the epidemiological investigation of pathogens of public health importance. However, whole-genome sequencing (WGS) of bacteria typically relies on culture, which represents a major hurdle for generating such data for a wide range of species for which culture is challenging. In this study, we assessed the use of culture-free target-enrichment sequencing as a method for generating genomic data for two bacterial species: (1)

Indexed as

GenomicsPublic HealthAnimalsBacteriaHumansWhole Genome SequencingBacillus anthracisbacteriagenomicMycoplasma amphoriformeRNA baitstarget-enrichment sequencing

Identifiers

PMID35622897
PMCPMC9465068
OpenAlexW4281923035

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.