Evidence map›Paper›PMID 35622331›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

AIRR Community Guide to Planning and Performing AIRR-Seq Experiments.

Anne Eugster, Magnolia L Bostick, Nidhi Gupta, Encarnita Mariotti-Ferrandiz, Gloria Kraus, Wenzhao Meng, Cinque Soto, Johannes Trück, Ulrik Stervbo, Eline T Luning Prak and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. AI-Driven BCR Modeling for Precision Immunology.International journal of molecular sciences · 2026
    Review
  2. Challenges and future directions of AIRR-seq-based diagnostics.Immunoinformatics (Amsterdam, Netherlands) · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Anne EugsterCenter for Regenerative Therapies Dresden, Faculty of Medicine, TU Dresden, Dresden, Germany.ORCID 0000-0001-8009-5959
Magnolia L BostickTakara Bio USA, San Jose, CA, USA.ORCID 0000-0002-2449-4995
Nidhi GuptaTakara Bio USA, San Jose, CA, USA.ORCID 0000-0002-2347-9339
Encarnita Mariotti-FerrandizINSERM, Immunology-Immunopathology-Immunotherapy (i3), Sorbonne Université, Paris, France.ORCID 0000-0002-8770-0186
Gloria KrausCenter for Regenerative Therapies Dresden, Faculty of Medicine, TU Dresden, Dresden, Germany.
Wenzhao MengDepartment of Pathology and Laboratory Medicine, Perelman School of Medicine, The University of Pennsylvania, Philadelphia, PA, USA.
Cinque SotoVanderbilt Vaccine Center and Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN, USA.ORCID 0000-0002-3997-6217
Johannes TrückDivision of Immunology and Children's Research Center, University Children's Hospital Zurich, University of Zurich (UZH), Zurich, Switzerland.ORCID 0000-0002-0418-7381
Ulrik StervboCenter for Translational Medicine, Immunology, and Transplantation, Medical Department, and Immundiagnostik, Marien Hospital Herne, University Hospital of the Ruhr-University Bochum, Herne, Germany. ulrik.stervbo@elisabethgruppe.de.ORCID 0000-0002-2831-8868
Eline T Luning PrakDepartment of Pathology and Laboratory Medicine, Perelman School of Medicine, The University of Pennsylvania, Philadelphia, PA, USA. luning@pennmedicine.upenn.edu.ORCID 0000-0002-9478-9211
AIRR Community

Funding

University of Pennsylvania Clinical Autoimmunity Center of ExcellenceUM1AI144288 · NIAID · UNIVERSITY OF PENNSYLVANIA · PI BAR-OR, AMIT, PAYNE, AIMEE S · 2019 to 2023
$551k
NIAID NIH HHS UM1 AI144288
6 · The paper itself

Abstract

The development of high-throughput sequencing of adaptive immune receptor repertoires (AIRR-seq of IG and TR rearrangements) has provided a new frontier for in-depth analysis of the immune system. The last decade has witnessed an explosion in protocols, experimental methodologies, and computational tools. In this chapter, we discuss the major considerations in planning a successful AIRR-seq experiment together with basic strategies for controlling and evaluating the outcome of the experiment. Members of the AIRR Community have authored several chapters in this edition, which cover step-by-step instructions to successfully conduct, analyze, and share an AIRR-seq project.

Indexed as

High-Throughput Nucleotide SequencingReceptors, ImmunologicReceptors, ImmunologicAIRR-seqAntibodyImmune repertoireImmunoglobulinNext-generation sequencingT-cell receptorV(D)J recombination

Identifiers

PMID35622331
PMCPMC9761540

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.