Evidence map›Paper›PMID 35622146›Full record

ArticleCellular and molecular life sciences : CMLS2022

Discovery of surface biomarkers for cell mechanophenotype via an intracellular protein-based enrichment strategy.

Megan E Dempsey, Graylen R Chickering, Rafael D González-Cruz, Vera C Fonseca, Eric M Darling

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular and molecular life sciences : CMLS, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Megan E DempseyCenter for Biomedical Engineering, Brown University, Providence, RI, 02912, USA.
Graylen R ChickeringCenter for Biomedical Engineering, Brown University, Providence, RI, 02912, USA.
Rafael D González-CruzCenter for Biomedical Engineering, Brown University, Providence, RI, 02912, USA.
Vera C FonsecaDepartment of Pathology and Laboratory Medicine, Brown University, Providence, RI, 02912, USA.
Eric M DarlingCenter for Biomedical Engineering, Brown University, Providence, RI, 02912, USA. eric_darling@brown.edu.ORCID http://orcid.org/0000-0002-3932-8623
Brown University · US

Funding

Pilot Projects ProgramP30GM122732 · NIGMS · RHODE ISLAND HOSPITAL · PI FLEMING, BRADEN · 2017 to 2021
$6.6M
High-yield, lineage-specific enrichment of living mesenchymal stem cellsR01AR063642 · NIAMS · BROWN UNIVERSITY · PI DARLING, ERIC M · 2013 to 2018
$1.8M
Advancing the Research Careers of Women and PEERs in Brain ScienceR25NS124530 · NINDS · BROWN UNIVERSITY · PI CARLOS D AIZENMAN, Diane Lipscombe · 2022 to 2026
$1.3M
Adult Stem Cell Enrichment via Biomechanical CharacterizationR00AR054673 · NIAMS · BROWN UNIVERSITY · PI DARLING, ERIC M · 2009 to 2011
$829k
Adult Stem Cell Enrichment via Biomechanical CharacterizationK99AR054673 · NIAMS · DUKE UNIVERSITY · PI DARLING, ERIC M · 2007 to 2007
$81k
NIAMS NIH HHS K99 AR054673NIAMS NIH HHS R00 AR054673NIAMS NIH HHS R01 AR063642NIGMS NIH HHS P30 GM122732NIH HHS P30 GM122732NIH HHS R01 AR054673NINDS NIH HHS R25 NS124530
6 · The paper itself

Abstract

Cellular mechanophenotype is often a defining characteristic of conditions like cancer malignancy/metastasis, cardiovascular disease, lung and liver fibrosis, and stem cell differentiation. However, acquiring living cells based on mechanophenotype is challenging for conventional cell sorters due to a lack of biomarkers. In this study, we demonstrate a workflow for surface protein discovery associated with cellular mechanophenotype. We sorted heterogeneous adipose-derived stem/stromal cells (ASCs) into groups with low vs. high lamin A/C, an intracellular protein linked to whole-cell mechanophenotype. Proteomic data of enriched groups identified surface protein candidates as potential biochemical proxies for ASC mechanophenotype. Select surface biomarkers were used for live-cell enrichment, with subsequent single-cell mechanical testing and lineage-specific differentiation. Ultimately, we identified CD44 to have a strong inverse correlation with whole-cell elastic modulus, with CD44

Indexed as

AdipogenesisProteomicsBiomarkersCell DifferentiationMembrane ProteinsBiomarkersMembrane ProteinsAtomic force microscopyCD44Elastic modulusLaminMechanobiologyProteomics

Identifiers

PMID35622146
PMCPMC9239330
OpenAlexW4281853246

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.