ArticleBioengineering (Basel, Switzerland)2022
Self-Assembled Hydrogel Microparticle-Based Tooth-Germ Organoids.
Article in Bioengineering (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 27 citations in OpenAlex.
- Advances in Regenerative Dentistry: From Biological Mechanisms to Clinical Translation.Life (Basel, Switzerland) · 2026Review
- A Fully Defined GelMA-Based Matrix Allows Fine Tuning of Tissue-Relevant Biomechanical and Biochemical Cues for Organoid Culture.Advanced healthcare materials · 2026Article
- Stem cell-driven biomedical technologies for tooth regeneration: engineering scaffolds, organoid models, and molecular targeted strategies.Stem cell research & therapy · 2026Review
- Tooth-on-a-chip to engineer early dental epithelial-mesenchymal interaction.Materials today. Bio · 2025Article
- Harnessing Oxidized Alginate Microgels for Rapid and Self-Assembling Dental Tissue Organogenesis In Vitro and In Vivo.Small science · 2025Article
- Hierarchical Delivery of Anti-Inflammatory Compound and Stem Cells for Chronic Wounds.Pharmaceutics · 2025Article
- Three-Dimensional Models of the Dental Pulp: Bridging Fundamental Biology and Regenerative Therapy.International journal of molecular sciences · 2025Review
- Dual Delivery of Cells and Bioactive Molecules for Wound Healing Applications.Molecules (Basel, Switzerland) · 2025Article
- Expansion and Delivery of Human Chondrocytes on Gelatin-Based Cell Carriers.Gels (Basel, Switzerland) · 2025Article
- Microporous annealed particle hydrogels in cell culture, tissue regeneration, and emerging application in cancer immunotherapy.American journal of cancer research · 2025Review
- Potential Implications of Organoids in Dental Research.Frontiers in dentistry · 2025Review
- Organoids in the oral and maxillofacial region: present and future.International journal of oral science · 2024Review
- Optimized Adipogenic Differentiation and Delivery of Bovine Umbilical Cord Stem Cells for Cultivated Meat.Gels (Basel, Switzerland) · 2024Article
- Dentin Mechanobiology: Bridging the Gap between Architecture and Function.International journal of molecular sciences · 2024Review
- Human Mesenchymal Stem Cells on Size-Sorted Gelatin Hydrogel Microparticles Show Enhanced In Vitro Wound Healing Activities.Gels (Basel, Switzerland) · 2024Article
- Revolutionising oral organoids with artificial intelligence.Biomaterials translational · 2024Review
- Hydrogel Microparticles for Bone Regeneration.Gels (Basel, Switzerland) · 2023Review
- Complex in vitro Model: A Transformative Model in Drug Development and Precision Medicine.Clinical and translational science · 2023Review
- Dental Restorations.Bioengineering (Basel, Switzerland) · 2023Article
- Engineered organoids in oral and maxillofacial regeneration.iScience · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Here, we describe the characterization of tooth-germ organoids, three-dimensional (3D) constructs cultured in vitro with the potential to develop into living teeth. To date, the methods used to successfully create tooth organoids capable of forming functional teeth have been quite limited. Recently, hydrogel microparticles (HMP) have demonstrated utility in tissue repair and regeneration based on their useful characteristics, including their scaffolding ability, effective cell and drug delivery, their ability to mimic the natural tissue extracellular matrix, and their injectability. These outstanding properties led us to investigate the utility of using HMPs (average diameter: 158 ± 32 µm) derived from methacrylated gelatin (GelMA) (degree of substitution: 100%) to create tooth organoids. The tooth organoids were created by seeding human dental pulp stem cells (hDPSCs) and porcine dental epithelial cells (pDE) onto the HMPs, which provided an extensive surface area for the cells to effectively attach and proliferate. Interestingly, the cell-seeded HMPs cultured on low-attachment tissue culture plates with gentle rocking self-assembled into organoids, within which the cells maintained their viability and morphology throughout the incubation period. The self-assembled organoids reached a volume of ~50 mm
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.