Evidence map›Paper›PMID 35620068›Full record

ArticleSTAR protocols2022

Isolation and RNA sequencing of single nuclei from

Colleen N McLaughlin, Yanyan Qi, Stephen R Quake, Liqun Luo, Hongjie Li

Open access · goldAbstract read
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
2.6field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 34 citations in OpenAlex.

  1. Article
  2. Cell-type-specific circadian and light-responsive transcriptional dynamics in adultProceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  3. Article
  4. Intrinsic regulation of intestinal stem cell fate and homeostasis by Tet.bioRxiv : the preprint server for biology · 2026
    Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Hippo signaling regulates cuticle pigmentation and dopamine metabolism inbioRxiv : the preprint server for biology · 2025
    Article
  10. Review
  11. A single-nucleus transcriptomic atlas of the adultbioRxiv : the preprint server for biology · 2025
    Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Colleen N McLaughlinDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Yanyan QiHuffington Center on Aging, Baylor College of Medicine, Houston, TX 77030, USA.
Stephen R QuakeDepartment of Bioengineering and Applied Physics, Stanford University, Stanford, CA, USA.
Liqun LuoDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Hongjie LiHuffington Center on Aging, Baylor College of Medicine, Houston, TX 77030, USA.
Baylor College of Medicine · USHoward Hughes Medical Institute · USChan Zuckerberg Initiative (United States) · US

Funding

Assembly of the Central Olfactory Networks in DrosophilaR01DC005982 · NIDCD · STANFORD UNIVERSITY · PI LIQUN LUO · 2003 to 2026
$8.1M
Characterizing age-related decline of neural circuits by single-cell profilingR00AG062746 · NIA · BAYLOR COLLEGE OF MEDICINE · PI LI, HONGJIE · 2021 to 2023
$996k
Howard Hughes Medical InstituteNIA NIH HHS R00 AG062746NIDCD NIH HHS R01 DC005982
6 · The paper itself

Abstract

Many insect cells are encapsulated within the exoskeleton and cannot be dissociated intact, making them inaccessible to single-cell transcriptomic profiling. We have used single-nucleus RNA sequencing to extract transcriptomic information from multiple

Indexed as

DrosophilaGene Expression ProfilingAnimalsBase SequenceExome SequencingSequence Analysis, RNAGenomicsModel OrganismsRNAseqSequencing

Identifiers

PMID35620068
PMCPMC9127693
OpenAlexW4280653388

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.