ArticleNature chemical biology2022
Biosynthesis of cyanobacterin, a paradigm for furanolide core structure assembly.
Article in Nature chemical biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- A guide to Direct Pathway Cloning (DiPaC) for natural product discovery.Essays in biochemistry · 2026Review
- The Maculalactone Biosynthetic Gene Cluster, a Cryptic Furanolide Pathway Revealed inACS synthetic biology · 2026Article
- Microbial unusual gene clusters without prominent core enzymes: natural products, biosynthesis and genome mining.The Journal of antibiotics · 2026Review
- Green genes from blue greens: challenges and solutions to unlocking the potential of cyanobacteria in drug discovery.Natural product reports · 2026Review
- Mangrove cyanobacterial diversity as a source of bioactive natural products.Current research in microbial sciences · 2026Article
- Halogenation-Guided Chemical Screening Uncovers Cyanobacterin Analogues from the CyanobacteriumJournal of natural products · 2025Article
- A Chemo-Enzymatic Platform for Furanolide Synthesis and Functional Exploration.Journal of the American Chemical Society · 2025Article
- Structural insights into two thiamine diphosphate-dependent enzymes and their synthetic applications in carbon-carbon linkage reactions.Nature chemistry · 2025Article
- Synthetic Biology in Natural Product Biosynthesis.Chemical reviews · 2025Review
- Heterologous Expression and Optimization of Fermentation Conditions for Recombinant Ikarugamycin Production.Biotechnology and bioengineering · 2025Article
- Heterologous Expression of a Cryptic BGC fromACS synthetic biology · 2025Article
- Agricultural Pest Management: The Role of Microorganisms in Biopesticides and Soil Bioremediation.Plants (Basel, Switzerland) · 2024Review
- Review
- The Potential of Allelochemicals from Microalgae for Biopesticides.Plants (Basel, Switzerland) · 2023Review
- Discovery and Heterologous Expression of Microginins fromACS synthetic biology · 2022Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The γ-butyrolactone motif is found in many natural signaling molecules and other specialized metabolites. A prominent example is the potent aquatic phytotoxin cyanobacterin, which has a highly functionalized γ-butyrolactone core structure. The enzymatic machinery that assembles cyanobacterin and structurally related natural products (herein termed furanolides) has remained elusive for decades. Here, we elucidate the biosynthetic process of furanolide assembly. The cyanobacterin biosynthetic gene cluster was identified by targeted bioinformatic screening and validated by heterologous expression in Escherichia coli. Full functional evaluation of the recombinant key enzymes in vivo and in vitro, individually and in concert, provided in-depth mechanistic insights into a streamlined C-C bond-forming cascade that involves installation of compatible reactivity at seemingly unreactive Cα positions of amino acid precursors. Our work extends the biosynthetic and biocatalytic toolbox for γ-butyrolactone formation, provides a general paradigm for furanolide biosynthesis and sets the stage for their targeted discovery, biosynthetic engineering and enzymatic synthesis.
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