ArticleCommunications biology2022
Temperature sensitivity of DNA double-strand break repair underpins heat-induced meiotic failure in mouse spermatogenesis.
Article in Communications biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 42 citations in OpenAlex.
- Reconstruction of xenogeneic seminiferous tubule-like structures in mouse testis using cryopreserved rat testicular cells.iScience · 2026Article
- Reverse transcriptase inhibitors enable the generation of fertile spermatids from fetal mouse testes in vitro.Communications biology · 2026Article
- Heat shock proteins in male infertility: recent advances and clinical implications.Frontiers in cell and developmental biology · 2026Review
- A simplified two-dimensional culture system supports meiotic progression during mouse spermatogenesis in vitro.PloS one · 2026Article
- Reproductive physiological impacts of high ambient temperature on animals: the impaired testicular function and compromised sperm quality in C57BL/6 mice.Biological research · 2025Article
- A guide to heat shock factors as multifunctional transcriptional regulators.The FEBS journal · 2025Review
- Rim4-seeded stress granules connect temperature sensing to meiotic regulation.Nature communications · 2025Article
- The involvement of TRPV1 in the apoptosis of spermatogenic cells in the testis of mice with cryptorchidism.Cell death discovery · 2025Article
- Mechanisms underlining Kelp (Saccharina japonica) adaptation to relative high seawater temperature.BMC genomics · 2025Article
- Abnormal H3K27me3 underlies degenerative spermatogonial stem cells in cryptorchid testis.Development (Cambridge, England) · 2025Article
- Spermatocyte injection into meiotic oocytes rescues diplotene, but not pachytene, arrest in azoospermic mutant mice.Human reproduction open · 2025Article
- Temperature regulates negative supercoils to modulate meiotic crossovers and chromosome organization.Science China. Life sciences · 2024Article
- Evaluation of the scrotal thermal environment and the testicular sizes in prepubertal hemiscrotal agenesis without cryptorchidism.IJU case reports · 2024Article
- Atypical heat shock transcription factor HSF5 is critical for male meiotic prophase under non-stress conditions.Nature communications · 2024Article
- Heatwaves are detrimental to fertility in the viviparous tsetse fly.Proceedings. Biological sciences · 2024Article
- In vivo CRISPR screening directly targeting testicular cells.Cell genomics · 2024Article
- Improvements in in vitro spermatogenesis: oxygen concentration, antioxidants, tissue-form design, and space control.The Journal of reproduction and development · 2024Article
- Effect of Temperature on the Development of Stages of Spermatogenesis and the Functionality of Sertoli Cells In Vitro.International journal of molecular sciences · 2024Article
- Trpv4-mediated apoptosis of Leydig cells induced by high temperature regulates sperm development and motility in zebrafish.Communications biology · 2024Article
- Cell cycle regulation for meiosis in mammalian germ cells.The Journal of reproduction and development · 2023Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mammalian spermatogenesis is a heat-vulnerable process that occurs at low temperatures, and elevated testicular temperatures cause male infertility. However, the current reliance on in vivo assays limits their potential to detail temperature dependence and destructive processes. Using ex vivo cultures of mouse testis explants at different controlled temperatures, we found that spermatogenesis failed at multiple steps, showing sharp temperature dependencies. At 38 °C (body core temperature), meiotic prophase I is damaged, showing increased DNA double-strand breaks (DSBs) and compromised DSB repair. Such damaged spermatocytes cause asynapsis between homologous chromosomes and are eliminated by apoptosis at the meiotic checkpoint. At 37 °C, some spermatocytes survive to the late pachytene stage, retaining high levels of unrepaired DSBs but do not complete meiosis with compromised crossover formation. These findings provide insight into the mechanisms and significance of heat vulnerability in mammalian spermatogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.