Evidence map›Paper›PMID 35618322›Full record

ArticleJournal of veterinary science2022

A combined application of molecular docking technology and indirect ELISA for the serodiagnosis of bovine tuberculosis.

Shengnan Song, Qian Zhang, Hang Yang, Jia Guo, Mingguo Xu, Ningning Yang, Jihai Yi, Zhen Wang, Chuangfu Chen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of veterinary science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Shengnan Song *College of Animal Science and Technology, Shihezi University, Shihezi 832000, China.ORCID https://orcid.org/0000-0001-8799-1713
Qian Zhang *State Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832003, China.ORCID https://orcid.org/0000-0003-0109-9631
Hang Yang *College of Animal Science and Technology, Shihezi University, Shihezi 832000, China.ORCID https://orcid.org/0000-0002-6615-2352
Jia GuoCollege of Animal Science and Technology, Shihezi University, Shihezi 832000, China.ORCID https://orcid.org/0000-0002-6211-8456
Mingguo XuCollege of Animal Science and Technology, Shihezi University, Shihezi 832000, China.ORCID https://orcid.org/0000-0003-3871-065X
Ningning YangCollege of Animal Science and Technology, Shihezi University, Shihezi 832000, China.ORCID https://orcid.org/0000-0003-0020-7231
Jihai YiCollege of Animal Science and Technology, Shihezi University, Shihezi 832000, China.ORCID https://orcid.org/0000-0002-7027-7668
Zhen WangCollege of Animal Science and Technology, Shihezi University, Shihezi 832000, China.ORCID https://orcid.org/0000-0003-0427-0078
Chuangfu ChenCollege of Animal Science and Technology, Shihezi University, Shihezi 832000, China.ORCID https://orcid.org/0000-0003-4986-5163
Shihezi University · CNXinjiang Academy of Agricultural and Reclamation Science · CN

Funding

Corps Major Scientific and Technological Projects 2017AA003Key R&D Project of Hebei Province 21322912DNational Natural Key Research and Development Plan 2017YFD0500304
6 · The paper itself

Abstract

backgroundThere is an urgent need to find reliable and rapid bovine tuberculosis (bTB) diagnostics in response to the rising prevalence of bTB worldwide. Toll-like receptor 2 (TLR2) recognizes components of bTB and initiates antigen-presenting cells to mediate humoral immunity. Evaluating the affinity of antigens with TLR2 can form the basis of a new method for the diagnosis of bTB based on humoral immunity.

objectivesTo develop a reliable and rapid strategy to improve diagnostic tools for bTB.

methodsIn this study, we expressed and purified the sixteen bTB-specific recombinant proteins in

resultsThe results showed that interaction surface Cα-atom root mean square deviation of proteins (M1, M2, MPT70, MPT83)-TLR2 protein are less than 2.5 A, showing a high affinity. It is verified by clinical serum samples that MPT70, MPT83, MPT70-MPT83 showed good diagnostic potential for the detection of anti-bTB IgG and M1, M2 can replace the whole protein as the detection antigen.

conclusionsMolecular docking to evaluate the affinity of bTB protein and TLR2 combined with ELISA provides new insights for the diagnosis of bTB.

Indexed as

Cattle DiseasesTuberculosis, BovineAnimalsAntigens, BacterialCattleEnzyme-Linked Immunosorbent AssayEpitopesEscherichia coliMolecular Docking SimulationSerologic TestsTechnologyToll-Like Receptor 2Antigens, BacterialEpitopesToll-Like Receptor 2Bovine tuberculosisenzyme-linked immunosorbent assaymolecular dockingrecombinant proteinstoll-like receptor 2

Identifiers

PMID35618322
PMCPMC9149502
OpenAlexW4281715888

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.