Evidence map›Paper›PMID 35616855›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Production of Recombinant Proteins in Transgenic Tobacco Plants.

Tim H Szeto, Pascal M W Drake, Audrey Y-H Teh, Nicole Falci Finardi, Ashleigh G Clegg, Mathew J Paul, Rajko Reljic, Julian K-C Ma

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.1field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Tim H Szeto *Hotung Molecular Immunology Unit, St. George's University of London, Institute for Infection and Immunity, London, UK.
Pascal M W Drake *Hotung Molecular Immunology Unit, St. George's University of London, Institute for Infection and Immunity, London, UK. pdrake@sgul.ac.uk.
Audrey Y-H Teh *Hotung Molecular Immunology Unit, St. George's University of London, Institute for Infection and Immunity, London, UK.
Nicole Falci Finardi *Hotung Molecular Immunology Unit, St. George's University of London, Institute for Infection and Immunity, London, UK.
Ashleigh G CleggHotung Molecular Immunology Unit, St. George's University of London, Institute for Infection and Immunity, London, UK.
Mathew J PaulHotung Molecular Immunology Unit, St. George's University of London, Institute for Infection and Immunity, London, UK.
Rajko ReljicHotung Molecular Immunology Unit, St. George's University of London, Institute for Infection and Immunity, London, UK.
Julian K-C MaHotung Molecular Immunology Unit, St. George's University of London, Institute for Infection and Immunity, London, UK.
St George's, University of London · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nicotiana tabacum (the tobacco plant ) has numerous advantages for molecular farming, including rapid growth, large biomass and the possibility of both cross- and self-fertilization. In addition, genetic transformation and tissue culture protocols for regeneration of transgenic plants are well-established. Here, we describe the production of transgenic tobacco using Agrobacterium tumefaciens and the analysis of recombinant proteins, either in crude plant extracts or after purification, by enzyme-linked immunosorbent assays, sodium dodecyl sulfate polyacrylamide gel electrophoresis with western blotting and surface plasmon resonance.

Indexed as

Agrobacterium tumefaciensNicotianaBlotting, WesternPlants, Genetically ModifiedRecombinant ProteinsRecombinant ProteinsAgrobacterium tumefaciensELISANicotiana tabacumRecombinant proteinSDS PAGESurface plasmon resonanceTransgenicWestern blotting

Identifiers

PMID35616855
OpenAlexW4285027849

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.