Evidence map›Paper›PMID 35614456›Full record

ArticleThrombosis journal2022

An in situ inferior vena cava ligation-stenosis model to study thrombin generation rates with flow.

Wei Yin, Andrew Dimatteo, Andrew Kumpfbeck, Stephen Leung, Marina Fandaros, Bryan Musmacker, David A Rubenstein, Mary D Frame

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Thrombosis journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 92% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Wei YinDepartment of Biomedical Engineering, Stony Brook University, Bioengineering Building, Room 109, Stony Brook, NY, 11794, USA. wei.yin@stonybrook.edu.
Andrew DimatteoDepartment of Biomedical Engineering, Stony Brook University, Bioengineering Building, Room 109, Stony Brook, NY, 11794, USA.
Andrew KumpfbeckDepartment of Biomedical Engineering, Stony Brook University, Bioengineering Building, Room 109, Stony Brook, NY, 11794, USA.
Stephen LeungDepartment of Biomedical Engineering, Stony Brook University, Bioengineering Building, Room 109, Stony Brook, NY, 11794, USA.
Marina FandarosDepartment of Biomedical Engineering, Stony Brook University, Bioengineering Building, Room 109, Stony Brook, NY, 11794, USA.
Bryan MusmackerDepartment of Biomedical Engineering, Stony Brook University, Bioengineering Building, Room 109, Stony Brook, NY, 11794, USA.
David A RubensteinDepartment of Biomedical Engineering, Stony Brook University, Bioengineering Building, Room 109, Stony Brook, NY, 11794, USA.
Mary D FrameDepartment of Biomedical Engineering, Stony Brook University, Bioengineering Building, Room 109, Stony Brook, NY, 11794, USA.
Stony Brook University · US

Funding

American Heart Association 16GRNT30440004
6 · The paper itself

Abstract

backgroundBlood flow-induced shear stress affects platelet participation in coagulation and thrombin generation. We aimed to develop an in vivo model to characterize thrombin generation rates under flow.

methodsAn in situ inferior vena cava (IVC) ligation-stenosis model was established using C57BL/6 mice. Wild type C57BL/6 mice were fed normal chow diet for two weeks before experiments. On the day of experiments, mice were anesthetized, followed by an incision through the abdominal skin to expose the IVC, which was then ligated (followed by reperfusion through a stenosis for up to 2 h). IVC blood flow rate was monitored using a Transonic ultrasound flow meter. In sham animals, the IVC was exposed following the same procedure, but no ligation was applied. Thrombin generation following IVC ligation was estimated by measuring mouse plasma prothrombin fragment 1-2 concentration. Mouse plasma factor Va concentration was measured using phospholipids and a modified prothrombinase assay. Blood vessel histomorphology, vascular wall ICAM-1, von Willebrand Factor, tissue factor, and PECAM-1 expression were measured using immunofluorescence microscopy.

resultsIVC blood flow rate increased immediately following ligation and stenosis formation. Sizable clots formed in mouse IVC following ligation and stenosis formation. Both plasma factor Va and prothrombin fragment 1-2 concentration reduced significantly following IVC ligation/stenosis, while no changes were observed with ICAM-1, von Willebrand Factor, tissue factor and PECAM-1 expression.

conclusionClot formation was successful. However, the prothrombin-thrombin conversion rate constant in vivo cannot be determined as local thrombin and FVa concentration (at the injury site) cannot be accurately measured. Modification to the animal model is needed to further the investigation.

Indexed as

Inferior vena cavaLigationPlateletThrombin generation

Identifiers

PMID35614456
PMCPMC9131541
OpenAlexW4281488661

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.