ArticleScientific reports2022
Application of Box-Behnken design for optimization of phenolics extraction from Leontodon hispidulus in relation to its antioxidant, anti-inflammatory and cytotoxic activities.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 46 citations in OpenAlex.
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- Development of ambient-stable fish cake snacks: effects of extrusion-retort parameters on physical and microbial properties.Food science and biotechnology · 2026Article
- Process optimization for the extraction ofFood chemistry: X · 2025Article
- Metabolomic Profiling, Box-Behnken Design-Based Optimization of Ultrasonic Extraction, and Skin Anti-Aging Potential of the Green Husk ofMolecules (Basel, Switzerland) · 2025Article
- Green extraction of poplar type propolis: ultrasonic extraction parameters and optimization via response surface methodology.BMC chemistry · 2025Article
- Optimizing sorbitol double dehydration: A Box-Behnken design approach with commercial sulfonic acid resin.Heliyon · 2024Article
- InvestigatingHeliyon · 2024Article
- LC/MS/MS and GC/MS/MS metabolic profiling of Leontodon hispidulus, in vitro and in silico anticancer activity evaluation targeting hexokinase 2 enzyme.Scientific reports · 2024Article
- Optimization of oil yield of Pelargonium graveolens L'Hér using Box-Behnken design in relation to its antimicrobial activity and in silico study.Scientific reports · 2023Article
- Comparison of In Vitro Estrogenic Activity ofMolecules (Basel, Switzerland) · 2023Article
- Bioactivity and Component Analysis of Water Extract ofFoods (Basel, Switzerland) · 2022Article
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5 authors at 3 institutions in 1 country.
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Abstract
To the best of our knowledge, there have been no phytochemical studies concerning the wild plant Leontodon hispidulus Boiss. (Asteraceae). Optimization of the green extraction process of the plant aerial parts, identification of main phenolic compounds, evaluation of antioxidant, anti-inflammatory and anticancer activities of the optimized extract have been carried out. HPLC-analysis was performed using 95% ethanolic extract. 3-Level Box-Behnken Design was applied for optimization of extraction yield and total phenolic content using 3-factors (ethanol/water ratio, material/solvent ratio and extraction time). Antioxidant, anticancer and anti-inflammatory activities were evaluated by ABTS-assay, prostate and cervical carcinoma human cell lines and carrageenan-induced rat paw edema model, respectively. HPLC-analysis showed the presence of quercetin, rutin, kaempferol, chlorogenic and ρ-coumaric acids. Increasing both ethanol/water ratio and material/solvent ratio decreased the yield, while, it increased by prolongation of the extraction time. High material/solvent ratio increased the phenolic content. The optimized extract showed high total phenolic content (104.18 µg/mg) using 201 ml of 74.5% ethanol/water at 72 h and good biological activities. Antioxidant activity was found to be 41.89 mg Trolox-equivalent/gm, with 80% free radicals inhibition. For anti-inflammatory activity, 100 mg/kg of the extract inhibited the edema in rats by 83.5% after 4 h of carrageenan injection as compared to 81.7% inhibition by indomethacin. Prostate carcinoma cell line was more sensitive to the anticancer activity of the extract than cervical carcinoma cell line (IC
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