ArticleScientific reports2022
Proline confers acid stress tolerance to Bacillus megaterium G18.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Orthology-based comparative transcriptomic analysis reveals conserved osmoadaptive responses in Bacillus velezensis and Bacillus paralicheniformis.Archives of microbiology · 2026Article
- Proline protects Wickerhamomyces anomalus against ethanol‑induced damage during logarithmic growth: integrated physiological, transcriptomic, and metabolomic analyses.BMC microbiology · 2026Article
- Metagenomic and Culture-Based Insights into Salinity-Driven Bacterial Community Dynamics throughout Crude Oil-Degrading Enrichment Cultivation.Journal of microbiology and biotechnology · 2026Article
- Nature Already Did the Screening: Drought-Driven Rhizosphere Recruitment Enables Inoculant Discovery in Tomato and Reveals a Candidate NovelMicroorganisms · 2026Article
- Increased Drought Stress Tolerance in Maize Seeds byPlants (Basel, Switzerland) · 2026Article
- Characterization of bacterial endophytes isolated fromFrontiers in microbiomes · 2026Article
- Differential impacts of various plant growth-promoting and osmotic tolerant bacterial strains on proline and sugar accumulation to enhance stress adaptations in tea plants.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Zinc solubilizing rhizobacteria as bioinoculant for turmeric: transcriptomic insights into acid stress adaptation, zinc mobilization and growth promotion.World journal of microbiology & biotechnology · 2025Article
- Cellulase Production by Ultraviolet-Derived MutantInternational journal of molecular sciences · 2025Article
- Draft genome sequence and comparative genomic analysis of Halomonas salifodinae strain A2 isolated from the Zapotitlán Salinas Valley, Puebla, Mexico.Extremophiles : life under extreme conditions · 2025Article
- Whole-Genome Profiling of Endophytic Strain B.L.Ns.14 fromMicroorganisms · 2024Article
- An oxalate decarboxylase-like cupin domain containing protein is involved in imparting acid stress tolerance in Bacillus amyloliquefaciens MBNC.World journal of microbiology & biotechnology · 2024Article
- Genomic analysis of acid tolerance genes and deciphering the function ofFrontiers in microbiology · 2024Article
- Genomic insights into Bacillus subtilis MBB3B9 mediated aluminium stress mitigation for enhanced rice growth.Scientific reports · 2023Article
- Untargeted Metabolomics for Unraveling the Metabolic Changes in Planktonic and Sessile Cells ofAntibiotics (Basel, Switzerland) · 2023Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proline plays a multifunctional role in several organisms including bacteria in conferring protection under stress conditions. In this paper we report the role of proline in conferring acid tolerance to Bacillus megaterium G18. An acid susceptible mutant of B. megaterium G18 which required proline for its growth under acid stress condition was generated through Tn5 mutagenesis. Further, targeted inactivation of proC involved in osmo-adaptive proline synthesis in B. megaterium G18 resulted in the loss of ability of the bacterium to grow at low pH (pH 4.5). Exogenous supply of proline (1 mM) to the growth medium restored the ability of the mutant cells to grow at pH 4.5 which was not the same in case of other osmoprotectants tested. Proline was produced and secreted to extracellular medium by B. megaterium G18 when growing in low pH condition as evidenced by the use of Escherichia coli proline auxotrophs and HPLC analysis. Further, pHT01 vector based expression of full length proC gene in the ∆proC mutant cells restored the survival capacity of the mutant cells in acidic pH, suggesting that proline production is an important strategy employed by B. megaterium G18 to survive under acid stress induced osmotic stress.
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