ArticleNature communications2022
Functional and multiscale 3D structural investigation of brain tissue through correlative in vivo physiology, synchrotron microtomography and volume electron microscopy.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 42 citations in OpenAlex.
- Evolution of hierarchical phase-contrast tomography on the European Synchrotron beamlines BM05 and BM18: a whole adult human brain imaging case study.Journal of synchrotron radiation · 2026Article
- Critical point drying of brain tissue for X-ray phase-contrast imaging.Journal of synchrotron radiation · 2026Article
- Three-dimensional high-content imaging of unstained soft tissue with subcellular resolution using a laboratory-based X-ray microscope.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- 3D imaging of neuronal inclusions and protein aggregates in human neurodegeneration by multiscale x-ray phase-contrast tomography.Brain pathology (Zurich, Switzerland) · 2026Article
- FaXToR: the hard X-ray micro-tomography beamline at the Spanish synchrotron ALBA.Journal of synchrotron radiation · 2026Article
- Nondestructive X-ray tomography of brain tissue ultrastructure.Nature methods · 2025Article
- Cryo-CLXEM introduces cryo-SXT to bridge the resolution gap in cryo-CLEM.Communications biology · 2025Article
- Three-photon microscopy: an emerging technique for deep intravital brain imaging.Nature reviews. Neuroscience · 2025Review
- Experimental comparison of X-ray ptychographic and holographic nanotomography of metal-stained neuronal tissue.Optics express · 2025Article
- Integrated 3D imaging of FFPE lung tissue combining microCT, light and electron microscopy allows for contextualized ultrastructural and histological analysis.Scientific reports · 2025Article
- Functional mapping of the molluscan brain guided by synchrotron X-ray tomography.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- tUbeNet: a generalizable deep learning tool for 3D vessel segmentation.Biology methods & protocols · 2025Article
- Synchrotron X-ray imaging of soft biological tissues - principles, applications and future prospects.Journal of cell science · 2024Review
- Revealing the MRI-Contrast in Optically Cleared Brains.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Between neurons and networks: investigating mesoscale brain connectivity in neurological and psychiatric disorders.Frontiers in neuroscience · 2024Review
- Current Best Practices for Analysis of Dendritic Spine Morphology and Number in Neurodevelopmental Disorder Research.ACS chemical neuroscience · 2023Review
- Femtosecond laser preparation of resin embedded samples for correlative microscopy workflows in life sciences.Applied physics letters · 2023Article
- Structural and functional imaging of brains.Science China. Chemistry · 2023Review
- Crosshair, semi-automated targeting for electron microscopy with a motorised ultramicrotome.eLife · 2022Article
- Volume electron microscopy.Nature reviews. Methods primers · 2022Article
Corrections and comments
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Authors and funding
15 authors at 5 institutions in 4 countries.
Funding
Abstract
Understanding the function of biological tissues requires a coordinated study of physiology and structure, exploring volumes that contain complete functional units at a detail that resolves the relevant features. Here, we introduce an approach to address this challenge: Mouse brain tissue sections containing a region where function was recorded using in vivo 2-photon calcium imaging were stained, dehydrated, resin-embedded and imaged with synchrotron X-ray computed tomography with propagation-based phase contrast (SXRT). SXRT provided context at subcellular detail, and could be followed by targeted acquisition of multiple volumes using serial block-face electron microscopy (SBEM). In the olfactory bulb, combining SXRT and SBEM enabled disambiguation of in vivo-assigned regions of interest. In the hippocampus, we found that superficial pyramidal neurons in CA1a displayed a larger density of spine apparati than deeper ones. Altogether, this approach can enable a functional and structural investigation of subcellular features in the context of cells and tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.