ArticlePloS one2022
Proteomic and microbiota analyses of the oral cavity during psychological stress.
Article in PloS one, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 13 citations in OpenAlex.
- Rapid shift of gut microbiome and enrichment of beneficial microbes during arhatic yoga meditation retreat in a single-arm pilot study.BMC complementary medicine and therapies · 2025Trial
- The oral microbiome is associated with stress, adversity, and mental health in young adults.Scientific reports · 2026Article
- Exploring the Role of Oral Microbiota in the Pathophysiology and Treatment of Bruxism.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Review
- Effects of psychosocial stress on laryngeal microbiology and epithelial barrier integrity.Scientific reports · 2025Article
- A New Way to Model Periodontitis in Laboratory Animals.Dentistry journal · 2023Article
- Psychological Functioning of Women Diagnosed with Lichen Planus and Other Diseases of the Oral Cavity-Explorative Study.Healthcare (Basel, Switzerland) · 2023Article
- Effect of psychological stress on the oral-gut microbiota and the potential oral-gut-brain axis.The Japanese dental science review · 2022Review
- Dual roles of ANGPTL4 in multiple inflammatory responses in stomatitis mice.Molecular biology reports · 2022Article
- Effects ofIn vivo (Athens, Greece)Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Psychological stress is associated with various oral diseases such as aphthous stomatitis, oral lichen planus, taste disturbances and glossodynia. However, the underlying mechanism is still unknown. The aim of this study was to determine the effect of psychological stress on salivary proteins and the oral microbiota in a rat model of chronic restraint stress. Six-week-old Sprague Dawley rats were subjected to restraint stress for four hours daily for 1 month. The behavior, weights of the adrenal glands, and serum corticosterone levels were evaluated as stress markers. Proteomic analysis of the saliva was performed using two-dimensional gel electrophoresis followed by mass spectrometry and Western blotting. Analysis of the oral microbiota was performed via 16S rRNA next-generation sequencing. The low mean body weights, lower number of entries and time spent in the open arm of elevated plus maze, high adrenal gland/body weight ratios, and high serum corticosterone levels confirmed the high levels of stress in the stress group of rats compared to the controls. Thirty-three protein spots were found to be significantly altered between the two groups. After silver staining, seven visible spots were subjected for mass spectrometry, and the expression levels of the two most significantly altered proteins, BPI fold containing family A member 2 and von Ebner's gland protein, were confirmed by Western blotting. 16S rRNA sequencing analysis revealed a significant reduction in alpha diversity in the stress group compared to the controls. The abundances of oral bacteria, such as Facklamia and Corynebacterium, were significantly altered between the two groups. Additionally, analysis with PICRUSt2 software predicted 37 different functional pathways to be altered between the groups. In conclusion, the present study identified altered salivary proteins and oral microbiota due to psychological stress. These findings might aid in understanding the pathogenesis of stress-related oral diseases.
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Registered trials
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