ArticleThe New phytologist2022
The tomato cytochrome P450 CYP712G1 catalyses the double oxidation of orobanchol en route to the rhizosphere signalling strigolactone, solanacol.
Article in The New phytologist, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 39 citations in OpenAlex.
- Strigolactones as Integrative Regulators of Plant Adaptation and Resilience to Abiotic Stress.Plant, cell & environment · 2026Review
- The chemical interaction between plants and the rhizosphere microbiome.Trends in plant science · 2025Review
- Engineering strigolactone signaling: toward crops that resist parasites without sacrificing symbiosis.Crop health · 2025Article
- Manipulation of a strigolactone transporter in tomato confers resistance to the parasitic weed broomrape.Innovation (Cambridge (Mass.)) · 2025Article
- Genome-wide identification and comparative analysis of strigolactone biosynthetic genes in major solanaceous crops.PloS one · 2025Article
- Chemistry and chemical biology tools contributing to the discovery and functional characterization of strigolactones.Frontiers in plant science · 2025Review
- OsCYP706C2 diverts rice strigolactone biosynthesis to a noncanonical pathway branch.Science advances · 2024Article
- Insights into stereoselective ring formation in canonical strigolactone: Identification of a dirigent domain-containing enzyme catalyzing orobanchol synthesis.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Tomato Mutants Reveal Root and Shoot Strigolactone Involvement in Branching and Broomrape Resistance.Plants (Basel, Switzerland) · 2024Article
- Cracking the enigma: understanding strigolactone signalling in the rhizosphere.Journal of experimental botany · 2024Review
- 2-oxoglutarate-dependent dioxygenases and BAHD acyltransferases drive the structural diversification of orobanchol in Fabaceae plants.Frontiers in plant science · 2024Article
- Disruption of the riceProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Strigolactones and Shoot Branching: What Is the Real Hormone and How Does It Work?Plant & cell physiology · 2023Review
- Article
- High-density linkage mapping and genetic dissection of resistance to broomrape (Frontiers in plant science · 2023Article
- Strigolactones as a hormonal hub for the acclimation and priming to environmental stress in plants.Plant, cell & environment · 2022Review
- Identification of novel canonical strigolactones produced by tomato.Frontiers in plant science · 2022Article
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Authors and funding
13 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Strigolactones (SLs) are rhizosphere signalling molecules and phytohormones. The biosynthetic pathway of SLs in tomato has been partially elucidated, but the structural diversity in tomato SLs predicts that additional biosynthetic steps are required. Here, root RNA-seq data and co-expression analysis were used for SL biosynthetic gene discovery. This strategy resulted in a candidate gene list containing several cytochrome P450s. Heterologous expression in Nicotiana benthamiana and yeast showed that one of these, CYP712G1, can catalyse the double oxidation of orobanchol, resulting in the formation of three didehydro-orobanchol (DDH) isomers. Virus-induced gene silencing and heterologous expression in yeast showed that one of these DDH isomers is converted to solanacol, one of the most abundant SLs in tomato root exudate. Protein modelling and substrate docking analysis suggest that hydroxy-orbanchol is the likely intermediate in the conversion from orobanchol to the DDH isomers. Phylogenetic analysis demonstrated the occurrence of CYP712G1 homologues in the Eudicots only, which fits with the reports on DDH isomers in that clade. Protein modelling and orobanchol docking of the putative tobacco CYP712G1 homologue suggest that it can convert orobanchol to similar DDH isomers as tomato.
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