Evidence map›Paper›PMID 35612754›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Untargeted Metabolomic Profiling of Fungal Species Populations.

Thomas E Witte, David P Overy

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.5field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Chemodiversity ofStudies in mycology · 2025
    Article
  3. CRISPR-Cas9 Gene Editing and Secondary Metabolite Screening ConfirmJournal of fungi (Basel, Switzerland) · 2023
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

Thomas E WitteAgriculture and Agri-Food Canada, Ottawa Research and Development Centre, Ottawa, ON, Canada.
David P OveryAgriculture and Agri-Food Canada, Ottawa Research and Development Centre, Ottawa, ON, Canada. david.overy@agr.gc.ca.
Agriculture and Agri-Food Canada · CAOttawa Research and Development Centre

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This chapter describes protocols for the development of consensus chemical phenotypes or "metabolomes" of fungal populations using ultra-high pressure liquid chromatography coupled to high resolution mass spectrometry (UPLC-HRMS). Isolates are cultured using multiple media conditions to elicit the expression of diverse secondary metabolite biosynthetic gene clusters. The mycelium and spent culture media are extracted using organic solvents and profiled by ultra-high pressure chromatography coupled with a high resolution Thermo Orbitrap XL mass spectrometer with the ability to trap and fragment ions to general MS

Indexed as

MetabolomeMetabolomicsChromatography, High Pressure LiquidMass SpectrometrySoftwareConsensus chemical phenotypesFungal natural productsFungiLCMSMass spectrometryMetabolomicsMZMineSecondary metabolitesThermo Orbitrap XLUPLC-HRMS

Identifiers

PMID35612754
OpenAlexW4285250247

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.