Evidence map›Paper›PMID 35612748›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Affinity Enrichment of Salmonella-Modified Membranes from Murine Macrophages for Proteomic Analyses.

Tzu-Chiao Chao, Samina Thapa, Nicole Hansmeier

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Tzu-Chiao ChaoDepartment of Biology, University of Regina, Regina, SK, Canada.
Samina ThapaDepartment of Biology, University of Regina, Regina, SK, Canada.
Nicole HansmeierDepartment of Biology, University of Regina, Regina, SK, Canada. Nicole.Hansmeier@uregina.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Dissecting host-pathogen interaction requires the ability to specifically enrich distinct proteins along with their co-assembled constituents or complexes. Affinity technologies leverage specificity of reagents to desired targets and help to enrich proteins of interests along with specifically associated proteins. Coupled with mass-spectrometry-based proteomics, this technology has become a powerful tool to explore pathogen compartments of diverse facultative and obligate intracellular pathogens. Here, we describe the process from infection of macrophages with Salmonella enterica to the affinity enrichment of Salmonella-modified membranes from murine macrophages.

Indexed as

ProteomicsSalmonella entericaAnimalsHost-Pathogen InteractionsMacrophagesMass SpectrometryMiceProteinsProteinsAffinity enrichmentHost–pathogen interactionImmunoprecipitationMacrophagesPathogen compartmentSalmonella enterica

Identifiers

PMID35612748

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.