Evidence map›Paper›PMID 35612738›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

HUNTER: Sensitive Automated Characterization of Proteolytic Systems by N Termini Enrichment from Microscale Specimen.

Anuli C Uzozie, Janice Tsui, Philipp F Lange

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.0field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Anuli C UzozieDepartment of Pathology, University of British Columbia, Vancouver, BC, Canada.
Janice TsuiDepartment of Pathology, University of British Columbia, Vancouver, BC, Canada.
Philipp F LangeDepartment of Pathology, University of British Columbia, Vancouver, BC, Canada. philipp.lange@ubc.ca.
University of British Columbia · CABC Children's Hospital · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Proteolysis occurs at low frequency in the cellular environment. Protein N termini reveal essential mechanisms associated with cellular functions, and are useful indicators to track dysfunctional regulation of proteins and pathways in diseases. N terminomics has so far relied on labor-intensive methods, which require relatively large starting sample amounts rendering it ill-suited for high-throughput systems biology studies. Here, we describe protocols for the first scalable and automatable method for sensitive enrichment and identification of N termini from minute samples.

Indexed as

ProteomeProteomicsPeptide HydrolasesProtein Processing, Post-TranslationalProteolysisPeptide HydrolasesProteomeN terminiProteoformProteolysis

Identifiers

PMID35612738
OpenAlexW4285231030

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.