Isolation of Detergent Insoluble Proteins from Mouse Brain Tissue for Quantitative Analysis Using Data Independent Acquisition (DIA).

Cristen Molzahn, Lorenz Nierves, Philipp F Lange, Thibault Mayor

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper, 1 of them a synthesis that pooled it.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed, 1 pooled it

1.9field-weighted citation impact, top 17% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 synthesis or guideline pooled it, 3 citations in OpenAlex.

Shift of the insoluble content of the proteome in the aging mouse brain.Proceedings of the National Academy of Sciences of the United States of America · 2023
Pooled it

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Cristen MolzahnMichael Smith Laboratories, Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, Canada.

Lorenz NiervesDepartment of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada.

Philipp F LangeDepartment of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada.

Thibault MayorMichael Smith Laboratories, Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, Canada. mayor@mail.ubc.ca.

BC Children's Hospital · CACanada's Michael Smith Genome Sciences Centre · CAUniversity of British Columbia · CA

Funding

CIHR PJT-148489

6 · The paper itself

Abstract

Enrichment of detergent insoluble proteins is a commonly used technique for analyzing proteins that may be aggregating in disease or with age. However, various methods for enriching for these proteins are used. Here we present a method using a mild detergent (Triton X-100) and high centrifugation speed (20,000 × g) allowing for sufficient protein extraction and enrichment for large protein assemblies. Digestion is performed on columns allowing for a methanol chloroform wash to remove the highly prevalent lipids in brain tissue. This is followed by analysis by data independent acquisition mass spectrometry, which we have found to be highly reproducible. Our method is intended to enrich for amorphous aggregates, which may accumulate upon the collapse of protein homeostasis.

Indexed as

BrainDetergentsAnimalsMass SpectrometryMiceOctoxynolProteinsDetergentsOctoxynolProteinsAgingMass spectrometryNeurodegenerative diseasesProtein aggregation

Identifiers

PMID35612733

DOI10.1016/j.jprot.2014.10.011

OpenAlexW4285180054

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.