Evidence map›Paper›PMID 35612314›Full record

ArticleMicrobiology spectrum2022

Rapid Hypothesis Testing in Candida albicans Clinical Isolates Using a Cloning-Free, Modular, and Recyclable System for CRISPR-Cas9 Mediated Mutant and Revertant Construction.

Junyan Liu, Amanda K Vogel, Jian Miao, Jennifer A Carnahan, David J Lowes, Jeffrey M Rybak, Brian M Peters

Open access · goldAbstract read
In one paragraph

Article in Microbiology spectrum, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
1.7field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 18 citations in OpenAlex.

  1. Article
  2. Phenotypic and genotypic analysis ofInfection and immunity · 2026
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  4. Loss of Fgr41 inInfection and immunity · 2026
    Article
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  9. Disruption tomSphere · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Junyan Liu *Department of Clinical Pharmacy and Translational Science, College of Pharmacy, University of Tennessee Health Science Centergrid.267301.1, Memphis, Tennessee, USA.
Amanda K Vogel *Integrated Program in Biomedical Sciences, College of Graduate Health Sciences, University of Tennessee Health Science Centergrid.267301.1, Memphis, Tennessee, USA.
Jian MiaoGraduate Program in Pharmaceutical Sciences, College of Graduate Health Sciences, University of Tennessee Health Science Centergrid.267301.1, Memphis, Tennessee, USA.
Jennifer A CarnahanDepartment of Clinical Pharmacy and Translational Science, College of Pharmacy, University of Tennessee Health Science Centergrid.267301.1, Memphis, Tennessee, USA.
David J LowesDepartment of Clinical Pharmacy and Translational Science, College of Pharmacy, University of Tennessee Health Science Centergrid.267301.1, Memphis, Tennessee, USA.
Jeffrey M RybakPharmacy and Pharmaceutical Sciences Department, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.
Brian M PetersDepartment of Clinical Pharmacy and Translational Science, College of Pharmacy, University of Tennessee Health Science Centergrid.267301.1, Memphis, Tennessee, USA.ORCID 0000-0002-0445-2077
University of Tennessee Health Science Center · USSt. Jude Children's Research Hospital · US

Funding

Candidalysin: a key mediator of Candida vaginitis immunopathologyR01AI134796 · NIAID · UNIVERSITY OF TENNESSEE HEALTH SCI CTR · PI Brian M Peters · 2018 to 2026
$2.9M
Targeted and forward genetic approaches to decipher the pathogenesis of symptomatic vulvovaginal candidiasisR21AI141829 · NIAID · UNIVERSITY OF TENNESSEE HEALTH SCI CTR · PI PETERS, BRIAN M · 2019 to 2020
$418k
NIAID NIH HHS R01 AI134796NIAID NIH HHS R21 AI141829
6 · The paper itself

Abstract

As increasing evidence emerges that interstrain genetic diversity among Candida albicans clinical isolates underpins phenotypic variation compared to the reference isolate SC5314, new genetic tools are required to interrogate gene function across strain backgrounds. Here, the

Indexed as

Candida albicansCRISPR-Cas SystemsHomozygoteHygromycin BRibonucleoproteinsSequence DeletionHygromycin BRibonucleoproteinsCandidaCas9CRISPRgeneticsmolecular geneticsmolecular mycologymutantmycology

Identifiers

PMID35612314
PMCPMC9241802
OpenAlexW4281476025

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.