Evidence map›Paper›PMID 35612308›Full record

ArticleMicrobiology spectrum2022

Chemical Exploration of a Highly Selective Scaffold with Activity against Intracellular

Samuel Njikan, Sara Ahmed, Alyssa Manning, Divya Awasthi, Yulia Ovechkina, Sultan Chowdhury, Arielle Butts, Tanya Parish

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Samuel NjikanInfectious Disease Research Institute, Seattle, Washington, USA.
Sara AhmedInfectious Disease Research Institute, Seattle, Washington, USA.
Alyssa ManningInfectious Disease Research Institute, Seattle, Washington, USA.
Divya AwasthiInfectious Disease Research Institute, Seattle, Washington, USA.
Yulia OvechkinaInfectious Disease Research Institute, Seattle, Washington, USA.
Sultan ChowdhuryInfectious Disease Research Institute, Seattle, Washington, USA.
Arielle ButtsInfectious Disease Research Institute, Seattle, Washington, USA.
Tanya ParishInfectious Disease Research Institute, Seattle, Washington, USA.ORCID 0000-0001-7507-0423
Infectious Disease Research Institute · US

Funding

High Content Screening of Mycobacterium TuberculosisR01AI132634 · NIAID · SEATTLE CHILDREN'S HOSPITAL · PI PARISH, TANYA · 2017 to 2019
$1.9M
NIAID NIH HHS R01 AI132634
6 · The paper itself

Abstract

We previously identified a phenylthiourea series with activity against intracellular Mycobacterium tuberculosis using a high-throughput, high-content assay. We conducted a catalog structure-activity relationship study with a collection of 35 analogs. We identified several thiourea derivatives with excellent potency against intracellular bacteria and good selectivity over eukaryotic cells. Compounds had much lower activity against extracellular bacteria, which was not increased by using cholesterol as the sole carbon source. Compounds were equally active against strains with mutations in QcrB or MmpL3, thereby excluding common, promiscuous targets as the mode of action. The phenylthiourea series represents a good starting point for further exploration to develop novel antitubercular agents.

Indexed as

Mycobacterium tuberculosisTuberculosis, Lymph NodeTuberculosis, Multidrug-ResistantAntitubercular AgentsHumansMicrobial Sensitivity TestsPhenylthioureaAntitubercular AgentsPhenylthioureaantibacterial drug discoveryhigh-content analysisMycobacterium tuberculosisphenotypic screening

Identifiers

PMID35612308
PMCPMC9241686
OpenAlexW4281479563

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.