Evidence map›Paper›PMID 35610430›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Recording of Diurnal Gene Expression in Peripheral Organs of Mice Using the RT-Biolumicorder.

Georgia Katsioudi, Alejandro Osorio-Forero, Flore Sinturel, Claudia Hagedorn, Florian Kreppel, Ueli Schibler, David Gatfield

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. A conditionalScience advances · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Georgia KatsioudiCenter for Integrative Genomics, University of Lausanne, Lausanne, Switzerland.
Alejandro Osorio-ForeroDepartment of Fundamental Neurosciences, University of Lausanne, Lausanne, Switzerland.
Flore SinturelDivision of Endocrinology, Diabetes, Nutrition and Patient Education, Department of Medicine, University Hospital of Geneva, Geneva, Switzerland.
Claudia HagedornBiochemistry and Molecular Medicine, Center for Biomedical Education and Research (ZBAF), Faculty of Health, Witten/Herdecke University, Witten, Germany.
Florian KreppelBiochemistry and Molecular Medicine, Center for Biomedical Education and Research (ZBAF), Faculty of Health, Witten/Herdecke University, Witten, Germany.
Ueli SchiblerDepartment of Molecular Biology, Faculty of Sciences, University of Geneva, Geneva, Switzerland.
David GatfieldCenter for Integrative Genomics, University of Lausanne, Lausanne, Switzerland. david.gatfield@unil.ch.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

There is high interest in investigating the daily dynamics of gene expression in mammalian organs, for example, in liver. Such studies help to elucidate how and with what kinetics peripheral clocks integrate circadian signals from the suprachiasmatic nucleus, which harbors the circadian master pacemaker, with other systemic and environmental cues, such as those associated with feeding and hormones. Organ sampling around the clock, followed by the analysis of RNA and/or proteins, is the most commonly used procedure in assessing rhythmic gene expression. However, this method requires large cohorts of animals and is only applicable to behaviorally rhythmic animals whose phases are known. Real-time recording of gene expression rhythms using luciferase reporters has emerged as a powerful method to acquire continuous, high-resolution datasets from freely moving individual mice. Here, we share our experience and protocols with this technique, using the RT-Biolumicorder setup.

Indexed as

Circadian ClocksCircadian RhythmAnimalsGene ExpressionGene Expression RegulationLiverLuciferasesMammalsMiceSuprachiasmatic NucleusLuciferasesCircadian rhythmsFeeding rhythmsGene expression regulationLuciferaseMouse liverPeripheral oscillatorsReal-time bioluminescence recordingRT-BiolumicorderTranscription

Identifiers

PMID35610430

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.