ReviewAnalytical biochemistry2022
Multi-wavelength analytical ultracentrifugation of biopolymer mixtures and interactions.
Review in Analytical biochemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed.
- Mechanism of NanR transcriptional activation of sialic acid metabolism in Streptococcus pneumoniae.Nucleic acids research · 2026Article
- Analytical Ultracentrifugation in Aotearoa: A Brief History of the Technique and its Place Among Other Tools Used to Investigate Biomolecular Interactions.Journal of the Royal Society of New Zealand · 2026Review
- Integrative biophysical characterization of molecular interactions: A case study with the sfGFP-nanobody complex.Analytical biochemistry · 2025Article
- Analytical Ultracentrifugation to Assess the Quality of LNP-mRNA Therapeutics.International journal of molecular sciences · 2024Article
- Methods for the Design and Analysis of Analytical Ultracentrifugation Experiments.Current protocols · 2024Article
- Analytical Ultracentrifugation-Calibrated Anion-Exchange Chromatography for Sensitive and Intact Determination of Osteopontin in Infant Formula and Dairy Products.Journal of agricultural and food chemistry · 2023Article
- Characterization and quantification of adeno-associated virus capsid-loading states by multi-wavelength analytical ultracentrifugation with UltraScan.Nanomedicine (London, England) · 2023Article
- Comparison of three AUC techniques for the determination of the loading status and capsid titer of AAVs.European biophysics journal : EBJ · 2023Article
- Investigation of dynamic solution interactions between NET-1 and UNC-5B by multi-wavelength analytical ultracentrifugation.European biophysics journal : EBJ · 2023Article
- A new UltraScan module for the characterization and quantification of analytical buoyant density equilibrium experiments to determine AAV capsid loading.European biophysics journal : EBJ · 2023Article
- Determination of mRNA copy number in degradable lipid nanoparticles via density contrast analytical ultracentrifugation.European biophysics journal : EBJ · 2023Article
- Structure-function studies reveal ComEA contains an oligomerization domain essential for transformation in gram-positive bacteria.Nature communications · 2022Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Multi-wavelength analytical ultracentrifugation (MW-AUC) is a recent development made possible by new analytical ultracentrifuge optical systems. MW-AUC extends the basic hydrodynamic information content of AUC and provides access to a wide range of new applications for biopolymer characterization, and is poised to become an essential analytical tool to study macromolecular interactions. It adds an orthogonal spectral dimension to the traditional hydrodynamic characterization by exploiting unique chromophores in analyte mixtures that may or may not interact. Here we illustrate the utility of MW-AUC for experimental investigations where the benefit of the added spectral dimension provides critical information that is not accessible, and impossible to resolve with traditional AUC methods. We demonstrate the improvements in resolution and information content obtained by this technique compared to traditional single- or dual-wavelength approaches, and discuss experimental design considerations and limitations of the method. We further address the advantages and disadvantages of the two MW optical systems available today, and the differences in data analysis strategies between the two systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.