Evidence map›Paper›PMID 35608349›Full record

ArticleMicrobiology spectrum2022

Virulence Factor Genes and Cytotoxicity of

Ewa Zastempowska, Magdalena Twarużek, Jan Grajewski, Henryka Lassa

Open access · goldAbstract read
In one paragraph

Article in Microbiology spectrum, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed, 1 pooled it
2.8field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 1 synthesis or guideline pooled it, 18 citations in OpenAlex.

  1. Veterinary macrolides againstFrontiers in veterinary science · 2026
    Pooled it
  2. Article
  3. Article
  4. Article
  5. Article
  6. Dairy FarmPathogens (Basel, Switzerland) · 2026
    Article
  7. Article
  8. Article
  9. PlyCYU endolysin targetingApplied and environmental microbiology · 2025
    Article
  10. Article
  11. Genomic Diversity, Virulome, and Resistome ofPathogens (Basel, Switzerland) · 2025
    Article
  12. Article
  13. Review
  14. Characterization of Virulence Factors and Antimicrobial Susceptibility ofAnimals : an open access journal from MDPI · 2024
    Article
  15. Article
  16. Evaluation ofVeterinarni medicina · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Ewa ZastempowskaDepartment of Physiology and Toxicology, Faculty of Biological Sciences, Kazimierz Wielki University, Bydgoszcz, Poland.ORCID 0000-0002-6667-3756
Magdalena TwarużekDepartment of Physiology and Toxicology, Faculty of Biological Sciences, Kazimierz Wielki University, Bydgoszcz, Poland.
Jan GrajewskiDepartment of Physiology and Toxicology, Faculty of Biological Sciences, Kazimierz Wielki University, Bydgoszcz, Poland.
Henryka LassaUniversity of Bydgoszcz, Bydgoszcz, Poland.
Kazimierz Wielki University in Bydgoszcz · PLUniversity of Bydgoszcz · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Streptococcus agalactiae can produce a wide variety of virulence factors, including toxins and proteins which facilitate adhesion to and colonization and invasion of the host cells. There are few reports on the characteristics of field isolates from bovine mastitis in Poland. Thus, the aim of this study was to determine the occurrence of types of hemolysis on blood agar, virulence factor genes, and cytotoxicity of S. agalactiae isolates derived from cows with mastitis across Poland. The study included 68 isolates. Virulence genes were tested using standard PCR, and cytotoxicity was determined using methylthiazol tetrazolium (MTT) and lactate dehydrogenase (LDH) tests. Among the tested isolates, 89.7% were β-hemolytic, 8.8% γ-hemolytic, and 1.5% alpha-hemolytic. The only genes detected in all isolates were the

Indexed as

Mastitis, BovineStreptococcal InfectionsAnimalsCattleChlorocebus aethiopsFemalePolandStreptococcus agalactiaeVero CellsVirulence FactorsVirulence Factorsin vitro cytotoxicityLDH testMTT testvirulence genes

Identifiers

PMID35608349
PMCPMC9241884
OpenAlexW4281397038

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.